广西猪源致病性大肠杆菌对抗菌药物的耐药性变化分析

发布时间：2018-12-23 08:52

【摘要】：【目的】分析2007和2016年广西猪源致病性大肠杆菌对抗菌药物的耐药性差异,为兽医临床用药防治猪大肠杆菌病提供参考依据。【方法】2016年初从广西25个猪场采集腹泻仔猪的肠内容物、直肠棉拭子或肠系膜淋巴结等200份样品,分离大肠杆菌后进行致病力试验,选取103株致病性大肠杆菌用于体外药敏试验,将药敏试验结果与2007年的相关结果进行对比分析。【结果】从腹泻仔猪的体内共分离获得大肠杆菌178株,分离率为89%,其中有143株大肠杆菌可致试验小鼠死亡。与2007年相比,2016年广西猪源致病性大肠杆菌对50%的抗菌药物(9种)的敏感率上升,50%的抗菌药物的敏感率下降,上升最明显的为壮观霉素,升高34.9%,下降最明显的为氟苯尼考,降低46.9%;除头孢曲松和强力霉素的中介率与2007年相比基本无变化外,其余16种抗菌药物的中介率均降低,最明显的为头孢拉啶,降低55.2%;耐药率降低的抗菌药物只有阿米卡星、壮观霉素和强力霉素3种,降低12.3%~15.5%,耐药率升高的抗菌药物有15种,上升率为1.2%~62.1%,其中阿莫西林上升62.1%,氟苯尼考上升57.0%。耐药谱方面,2007年以5~11重耐药为主,占受试菌总数的75.7%,2016年以11重耐药以上为主,占74.8%。【结论】当前广西猪源致病性大肠杆菌以广谱耐药为主,敏感性降低,中介率降低,耐药率明显升高,生产中可选用阿米卡星和壮观霉素进行猪大肠杆菌病防治。
[Abstract]:[objective] to analyze the differences of antimicrobial resistance of pathogenic Escherichia coli from pigs in Guangxi in 2007 and 2016. [methods] 200 samples of intestinal contents, rectal cotton swabs and mesenteric lymph nodes of piglets with diarrhea were collected from 25 pig farms in Guangxi in early 2016. After isolation of Escherichia coli, the pathogenicity test was carried out. 103 strains of pathogenic Escherichia coli were selected for drug sensitivity test in vitro. Results 178 strains of Escherichia coli were isolated from diarrhea piglets, and the isolation rate was 89%. 143 strains of Escherichia coli could cause death in test mice. Compared with 2007, the susceptibility rate of pathogenic Escherichia coli to 50% antimicrobial agents (9 species) increased in 2016, and the sensitivity rate of 50% antimicrobial agents decreased. The most obvious increase was spectinomycin, which increased 34.9%. The most obvious decrease was florfenicol and decreased 46.9%. Except for ceftriaxone and doxycycline, the mediating rates of the other 16 antimicrobial agents were all decreased, the most obvious being cefradine, and the decrease of 55.2% was the most obvious, except that the mediating rate of ceftriaxone and doxycycline had no change compared with that of 2007. The antimicrobial resistance rate decreased only by amikacin, spectinomycin and doxycycline, and decreased by 12.3and 15.5. there were 15 antimicrobial agents with high resistance rate, and the rising rate was 1.2%. Amoxicillin rose 62.1 and florfenicol increased 57.0. In the aspect of drug resistance spectrum, the main drug resistance in 2007 was 5x11, which accounted for 75.7% of the total number of tested bacteria, and in 2016, the drug resistance was more than 11, accounting for 74.8%. [conclusion] at present, broad-spectrum resistance is the main cause of pathogenic Escherichia coli in Guangxi pigs, and the sensitivity is decreased. Amikacin and spectinomycin can be used to prevent and control colibacillosis in pigs.
【作者单位】：广西兽医研究所/广西兽医生物技术重点实验室;广西大学动物科学技术学院;
【基金】：广西科学研究与技术开发计划项目(桂科重14121003-4-1) 广西水产畜牧兽医局科技项目(桂渔牧科201528005)
【分类号】：S852.61

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