新城疫弱毒活疫苗中FAdV-4的分离鉴定及分子特征

发布时间：2018-12-25 17:53

【摘要】：【目的】当前我国鸡群禽腺病毒(Fowl adenovirus,FAd V)感染严重,该病毒既可水平传播也可经卵垂直传播,因此鸡胚源禽用弱毒活疫苗是否存在FAd V污染,进而造成疫病的传播尚不清楚。检测我国商品化新城疫La Sota弱毒活疫苗中是否存在FAd V污染,探讨国内鸡群中FAd V感染和流行的可能原因。【方法】应用本实验室建立的FAd V PCR检测方法检测商品化新城疫La Sota弱毒活疫苗,并进行FAd V分离鉴定。经序列比对和进化分析,鉴定分离毒株所属种、血清型及进化特征。【结果】分离到一株禽腺病毒,命名为L160962。基因分析表明,分离株L160962的52k和hexon基因片段序列与C种内的血清4型毒株相似性最高,其中与中国毒株HN/151025和CH/HNJZ/2015的相似性为99.9%-100%,与奥地利毒株KR5的相似性为99.3%,但与A、B、D和E种FAd V的相似性分别为79.0%-83.3%。系统进化分析表明,L160962与C种内的血清4型禽腺病毒在同一分支,核苷酸相似性在99.9%以上,应划分为血清4型FAd V。基于分离株L160962的hexon基因构建的系统进化树的拓扑结构,分离株L160962与中国毒株亲缘关系最近,提示疫苗源分离株L160962与我国当前流行毒株相近。【结论】我国商品化的新城疫La Sota弱毒活疫苗中存在血清4型FAd V污染,这可能是我国鸡群中FAd V大面积感染和流行的原因之一。
[Abstract]:[objective] at present, the avian adenovirus (Fowl adenovirus,FAd V) infection is serious in China. The virus can be transmitted both horizontally and vertically through eggs, so whether there is FAd V contamination in live vaccine of chicken embryo source poultry. The spread of the disease, in turn, is unclear. To detect the presence of FAd V pollution in commercial Newcastle disease (NDV) La Sota attenuated live vaccine in China. To explore the possible causes of FAd V infection and epidemic in domestic chickens. [methods] the commercial Newcastle disease La Sota attenuated vaccine was detected by FAd V PCR method established in our laboratory, and the FAd V was isolated and identified. By sequence alignment and evolutionary analysis, we identified the species, serotype and evolutionary characteristics of the isolated strain. [results] A avian adenovirus strain named L160962 was isolated. Gene analysis showed that the sequence of 52k and hexon gene fragments of strain L160962 had the highest similarity with serotype 4 of strain C, and the similarity with HN/151025 and CH/HNJZ/2015 of Chinese strain was 99.9- 100. The similarity with the Austrian strain KR5 was 99.3, but the similarity with FAd V was 79.0-83.3, respectively. Phylogenetic analysis showed that L160962 had the same branch with serotype 4 adenovirus, and the nucleotide similarity was more than 99.9%, which should be divided into serotype 4 FAd V. Based on the topological structure of phylogenetic tree constructed by hexon gene of isolate L160962, the phylogenetic relationship between L160962 and Chinese strain was close. The results suggest that the vaccine source strain L160962 is close to that of the current epidemic strain in China. [conclusion] serotype 4 FAd V contamination exists in the commercial Newcastle disease La Sota attenuated live vaccine in China. This may be one of the causes of FAd V infection and prevalence in Chinese chickens.
【作者单位】：东北农业大学动物科学技术学院;东北农业大学生命科学学院;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽传染病研究室;
【基金】：国家重点研发计划项目(No.2017YFD0500105) 国家科技支撑计划项目(No.2015BAD12B03) 中央级公益性科研院所基本科研业务费专项(No.1610302017003)~~
【分类号】：S858.31

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