仔猪A型口蹄疫母源抗体及细胞因子消长规律
发布时间：2018-12-26 15:54
【摘要】：口蹄疫(Foot-and-mouth disease,FMD)是由口蹄疫病毒(Foot-and-mouth disease virus,FMDV)感染引起的偶蹄动物的一种急性、热性、高度接触性传染病。该病多次在世界范围内爆发流行,造成巨大政治、经济损失。新生仔猪有自己的免疫系统,但不成熟,不能充分抵抗病原体感染。而母源抗体则为仔猪提供了早期保护作用。但母源抗体具有两面性,即能保护仔猪免受感染,又具有干扰疫苗免疫的作用。因此,探索猪口蹄疫母源抗体的传递方式,明确母源抗体在仔猪体内的消长规律,对于合理制定仔猪口蹄疫疫苗免疫程序具有重要意义。1.采用液相阻断ELISA和病毒中和试验检测5头母猪和25头仔猪血清中A型口蹄疫抗体水平。结果表明,仔猪采食初乳前血清中几乎不存在A型口蹄疫抗体,采食初乳1d后抗体水平达到峰值并在5~7d内维持稳定,而后逐渐降低,证明母源抗体是通过初乳传递给仔猪的。根据抗体水平和消减速度确定,母猪抗体水平为1:1024、1:720和1:256时,仔猪最宜首免时间分别为56、42和28日龄左右。2.采用ELISA检测分析母猪血清、乳汁及对应仔猪血清和脐带血中IgG、IgA、IgM的含量变化趋势。结果表明,母猪血清和初乳中3种免疫球蛋白含量丰富,仔猪采食初乳前的血清和脐带血中不含IgG、IgA、IgM,仔猪采食初乳1d后血清中IgG、IgM、IgA含量迅速增加,然后IgA和IgG含量呈连续下降趋势,而IgM含量持续下降至42d左右又会大幅度回升。3.采用抗体芯片QAP-CYT-1和QAP-CYT-2检测母猪血清、乳汁及对应仔猪血清中20种细胞因子。结果表明,母猪血清和乳汁中20种细胞因子含量丰富;其中8种细胞因子(TNFa、TGFb1、IFNg、GM-CSF、IL-10、IL-6、IL-4、IL-1b)在仔猪采食初乳前的血清中未检出,但在采食初乳后含量大幅度升高,说明这8种细胞因子是通过初乳被动传递的;其余12种细胞因子(CCL3L1、IFNα、IL-1α、IL-1ra、IL-13、IL-17A、MIG、IL-18、MIP-1b、PECEM-1、IL-8、IL-12P40P70)在仔猪采食初乳前的血清中就已经存在,仔猪采食初乳后其含量增加,说明这12种细胞因子也可以通过初乳传递给仔猪,但是否通过胎盘传递,尚需进一步证明。
[Abstract]:Foot and mouth disease (Foot-and-mouth disease,FMD) is an acute, feverish and highly contagious disease in cloven-hoofed animals caused by foot-and-mouth disease virus (Foot-and-mouth disease virus,FMDV). The disease broke out many times in the world, causing huge political and economic losses. Newborn piglets have their own immune system, but not mature enough to resist pathogen infection. Maternal antibodies provide early protection for piglets. But maternal antibody has two sides, it can protect piglets from infection and interfere with vaccine immunity. Therefore, it is of great significance to explore the transmission mode of maternal antibody against foot-and-mouth disease (FMD) in piglets and to clarify the rules of maternal antibody growth and decline in piglets, which is of great significance for the rational formulation of immunization procedures for foot-and-mouth disease vaccine in piglets. The level of FMD type A antibody in serum of 5 sows and 25 piglets was detected by liquid phase blocking ELISA and virus neutralization test. The results showed that there was almost no type A foot-and-mouth disease antibody in the serum of piglets before feeding colostrum, and the antibody level reached the peak level after 1 day of feeding colostrum and remained stable within 5 ~ 7 days, but gradually decreased. It was proved that maternal antibody was transmitted to piglets through colostrum. According to the antibody level and the subtractive speed, when the antibody level of sows was 1: 1024, 1: 720 and 1: 256, the optimal first immunity time of piglets was about 5642 and 28 days old, respectively. The changes of IgG,IgA,IgM content in serum, milk and corresponding piglets and umbilical cord blood were analyzed by ELISA. The results showed that the three kinds of immunoglobulin were abundant in serum and colostrum of sows, and the IgG,IgM,IgA content in serum and cord blood of piglets before feeding colostrum and in cord blood without IgG,IgA,IgM, increased rapidly after feeding colostrum for 1 day. Then the content of IgA and IgG decreased continuously, and the content of IgM continued to decrease to 42 days. QAP-CYT-1 and QAP-CYT-2 were used to detect 20 cytokines in sows' serum, milk and corresponding piglets. The results showed that 20 cytokines were abundant in serum and milk of sows. Eight kinds of cytokines (TNFa,TGFb1,IFNg,GM-CSF,IL-10,IL-6,IL-4,IL-1b) were not detected in the serum of piglets before feeding colostrum, but the contents increased significantly after feeding colostrum. These eight cytokines were transmitted passively through colostrum. The other 12 cytokines (CCL3L1,IFN 伪, IL-1 伪, IL-1ra,IL-13,IL-17A,MIG,IL-18,MIP-1b,PECEM-1,IL-8,IL-12P40P70) were already present in the serum of piglets before feeding colostrum. These 12 cytokines can also be transmitted to piglets through colostrum, but whether they are transmitted through placenta or not needs further proof.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.28
[Abstract]:Foot and mouth disease (Foot-and-mouth disease,FMD) is an acute, feverish and highly contagious disease in cloven-hoofed animals caused by foot-and-mouth disease virus (Foot-and-mouth disease virus,FMDV). The disease broke out many times in the world, causing huge political and economic losses. Newborn piglets have their own immune system, but not mature enough to resist pathogen infection. Maternal antibodies provide early protection for piglets. But maternal antibody has two sides, it can protect piglets from infection and interfere with vaccine immunity. Therefore, it is of great significance to explore the transmission mode of maternal antibody against foot-and-mouth disease (FMD) in piglets and to clarify the rules of maternal antibody growth and decline in piglets, which is of great significance for the rational formulation of immunization procedures for foot-and-mouth disease vaccine in piglets. The level of FMD type A antibody in serum of 5 sows and 25 piglets was detected by liquid phase blocking ELISA and virus neutralization test. The results showed that there was almost no type A foot-and-mouth disease antibody in the serum of piglets before feeding colostrum, and the antibody level reached the peak level after 1 day of feeding colostrum and remained stable within 5 ~ 7 days, but gradually decreased. It was proved that maternal antibody was transmitted to piglets through colostrum. According to the antibody level and the subtractive speed, when the antibody level of sows was 1: 1024, 1: 720 and 1: 256, the optimal first immunity time of piglets was about 5642 and 28 days old, respectively. The changes of IgG,IgA,IgM content in serum, milk and corresponding piglets and umbilical cord blood were analyzed by ELISA. The results showed that the three kinds of immunoglobulin were abundant in serum and colostrum of sows, and the IgG,IgM,IgA content in serum and cord blood of piglets before feeding colostrum and in cord blood without IgG,IgA,IgM, increased rapidly after feeding colostrum for 1 day. Then the content of IgA and IgG decreased continuously, and the content of IgM continued to decrease to 42 days. QAP-CYT-1 and QAP-CYT-2 were used to detect 20 cytokines in sows' serum, milk and corresponding piglets. The results showed that 20 cytokines were abundant in serum and milk of sows. Eight kinds of cytokines (TNFa,TGFb1,IFNg,GM-CSF,IL-10,IL-6,IL-4,IL-1b) were not detected in the serum of piglets before feeding colostrum, but the contents increased significantly after feeding colostrum. These eight cytokines were transmitted passively through colostrum. The other 12 cytokines (CCL3L1,IFN 伪, IL-1 伪, IL-1ra,IL-13,IL-17A,MIG,IL-18,MIP-1b,PECEM-1,IL-8,IL-12P40P70) were already present in the serum of piglets before feeding colostrum. These 12 cytokines can also be transmitted to piglets through colostrum, but whether they are transmitted through placenta or not needs further proof.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.28
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