体内法和体外法对玉米代谢能的评定效果比较
发布时间：2019-01-04 14:40
【摘要】：通过对体外法(仿生消化法)测定的玉米的酶水解物能值(EHGE)与体内法(动物试验法)测定的真代谢能(TME)进行比较,筛选快速、准确估测饲料能值的方法。体内法采用动物试验法进行代谢试验测定TME,选用504只21日龄AA肉仔鸡,按体质量均分为7个处理,每个处理12个重复,每个重复6只鸡,其中6个处理分别饲喂6个地区玉米饲料原料,另外1个处理为饥饿组,测定其内源能量代谢,试验期3 d。体外法采用仿生消化法进行仿生消化试验测定EHGE,6个地区玉米饲料原料设为6个处理,每个处理10个重复,每个重复2根消化管。结果表明:体外法测定的EHGE和体内法测定的TME总体上差异不显著。体外法测定的EHGE的变异系数在0.21%~0.87%,而体内法测定的TEM的变异系数在0.98%~4.12%,体外法测定的EHGE对体内法测定的TME的估计偏差均在2%以内,体外法测定的EHGE与体内法测定的TME呈显著线性关系(R2=0.95)。可见,用体外法测定的EHGE具有较高的准确性,且达到了估测体内法TME的要求,能快速、准确地评价日粮的代谢能。
[Abstract]:The enzyme hydrolytic energy (EHGE) of maize determined by bionic digestion in vitro was compared with the true metabolizable energy (TME) measured by in vivo method (animal test) to select a fast and accurate method for estimating feed energy value. In vivo Metabolism Test by Animal Test TME, 504 21-day-old AA broilers were divided into 7 treatments, 12 replicates per treatment, 6 chickens per replica, according to body mass. Among them, 6 treatments were fed with corn feed raw materials in 6 regions respectively, and the other one was treated with starvation group. The endogenous energy metabolism was measured for 3 days. In vitro bionic digestion test was used to determine the corn feed raw materials in 6 areas of EHGE, were treated with 6 treatments with 10 repeats each with 2 digestive tubes per repeat. The results showed that there was no significant difference between in vitro EHGE and in vivo TME. The coefficient of variation of EHGE measured by in vitro method was 0.21 and 0.87, while the coefficient of variation of TEM measured by in vivo method was 0.98 and 4.12.The estimated deviation of EHGE determined by in vitro method to TME determined by in vivo method was within 2%. There was a significant linear relationship between EHGE determined in vitro and TME measured in vivo (R _ 2O _ (0.95). It can be seen that the determination of EHGE by in vitro method has high accuracy and meets the requirement of estimating TME in vivo, which can quickly and accurately evaluate the metabolic energy of diet.
【作者单位】： 河南农业大学牧医工程学院;河南省农业科学院畜牧兽医研究所;
【基金】：国家现代农业产业技术体系项目(CARS-42) 河南省农业科学院自主创新专项
【分类号】：S816
本文编号：2400419
[Abstract]:The enzyme hydrolytic energy (EHGE) of maize determined by bionic digestion in vitro was compared with the true metabolizable energy (TME) measured by in vivo method (animal test) to select a fast and accurate method for estimating feed energy value. In vivo Metabolism Test by Animal Test TME, 504 21-day-old AA broilers were divided into 7 treatments, 12 replicates per treatment, 6 chickens per replica, according to body mass. Among them, 6 treatments were fed with corn feed raw materials in 6 regions respectively, and the other one was treated with starvation group. The endogenous energy metabolism was measured for 3 days. In vitro bionic digestion test was used to determine the corn feed raw materials in 6 areas of EHGE, were treated with 6 treatments with 10 repeats each with 2 digestive tubes per repeat. The results showed that there was no significant difference between in vitro EHGE and in vivo TME. The coefficient of variation of EHGE measured by in vitro method was 0.21 and 0.87, while the coefficient of variation of TEM measured by in vivo method was 0.98 and 4.12.The estimated deviation of EHGE determined by in vitro method to TME determined by in vivo method was within 2%. There was a significant linear relationship between EHGE determined in vitro and TME measured in vivo (R _ 2O _ (0.95). It can be seen that the determination of EHGE by in vitro method has high accuracy and meets the requirement of estimating TME in vivo, which can quickly and accurately evaluate the metabolic energy of diet.
【作者单位】： 河南农业大学牧医工程学院;河南省农业科学院畜牧兽医研究所;
【基金】：国家现代农业产业技术体系项目(CARS-42) 河南省农业科学院自主创新专项
【分类号】：S816
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