镰刀菌毒素污染饲粮导致的断奶仔猪肝脏损伤与自然修复研究

发布时间：2019-01-04 15:11

【摘要】：本试验旨在研究阶段性饲喂镰刀菌毒素污染饲粮对仔猪血清和肝脏生化指标、抗氧化指标以及肝脏炎性细胞因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)mRNA相对表达量的影响。选用体重为(8.45±0.94)kg的35日龄"杜×长×大"雌性仔猪30头,随机分为3组,每组10个重复,每个重复1头猪。对照组持续饲喂基础饲粮,镰刀菌毒素组持续饲喂镰刀菌毒素污染饲粮(玉米赤霉烯酮0.90 mg/kg,呕吐毒素1.43 mg/kg,烟曲霉毒素5.85 mg/kg),自然恢复组在饲喂镰刀菌毒素污染饲粮35 d后,改饲喂基础饲粮。预试期7 d,正试期56 d。结果表明:1)与对照组相比,镰刀菌毒素组仔猪肝脏相对重量显著升高(P0.05),血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(ALP)活性显著升高(P0.05),血清总蛋白(TP)和球蛋白(GLB)含量显著降低(P0.05),血清谷胱甘肽过氧化物酶(GSH-Px)、血清和肝脏总超氧化物歧化酶(T-SOD)活性显著下降(P0.05),血清和肝脏丙二醛(MDA)含量显著升高(P0.05);肝脏炎性细胞因子IL-1β和IL-6 mRNA相对表达量显著升高(P0.05)。2)经21 d自然恢复后,与镰刀菌毒素组相比,自然恢复组仔猪肝脏相对重量有所降低(P0.05),血清AST、ALT和ALP活性显著降低(P0.05),血清TP和GLB含量显著升高(P0.05),血清T-SOD活性显著升高(P0.05),血清和肝脏GSH-Px、肝脏T-SOD活性有所升高(P0.05),血清和肝脏M DA含量显著降低(P0.05),肝脏炎性细胞因子IL-1β和IL-6 mRNA相对表达量显著降低(P0.05)。由此可见,本试验条件下,长期饲喂镰刀菌毒素能够造成仔猪肝脏损伤,影响肝脏的抗氧化、蛋白质合成和免疫功能,而经过21 d恢复期,仔猪肝脏抗氧化和免疫功能得到了一定程度的改善。
[Abstract]:The aim of this study was to study the effects of periodic feeding of Fusarium toxin on serum and liver biochemical index, antioxidant index and hepatic inflammatory cytokine interleukin-1 尾 (IL-1 尾) in piglets. Effect of relative expression of interleukin-6 (IL-6) mRNA. Thirty 35 day old female piglets with body weight of (8.45 卤0.94) kg were randomly divided into 3 groups, with 10 replicates in each group and 1 pig per replica. The control group was continuously fed with basic diet, and the Fusarium toxin group was continuously fed with Fusarium toxin (0.90 mg/kg, vomit toxin 1.43 mg/kg, fumigatoxin 5.85 mg/kg). After feeding Fusarium toxin contaminated diet for 35 days, the natural recovery group was fed with basic diet. The pretest period was 7 days and the current test period was 56 days. The results showed that: 1) compared with the control group, the relative weight of liver in Fusarium toxin group was significantly higher than that in the control group (P0.05), and the serum alanine aminotransferase (ALT),) level was significantly increased. The activities of glutamic oxalate aminotransferase (AST) and alkaline phosphatase (ALP) increased significantly (P0.05), the contents of serum total protein (TP) and globulin (GLB) decreased significantly (P0.05), and the levels of serum glutathione peroxidase (GSH-Px), glutathione peroxidase (GSH-Px) in serum decreased significantly (P0.05). Serum and liver total superoxide dismutase (T-SOD) activity decreased significantly (P0.05), serum and liver malondialdehyde (MDA) content increased significantly (P0.05). The relative expression of IL-1 尾 and IL-6 mRNA in liver was significantly increased (P0.05). After 21 days of natural recovery, the relative weight of liver in natural recovery group was lower than that in Fusarium toxin group (P0.05). Serum AST,ALT and ALP activities decreased significantly (P0.05), serum TP and GLB levels increased significantly (P0.05), serum T-SOD activities increased significantly (P0.05), and serum and liver GSH-Px, levels increased significantly (P0.05). Liver T-SOD activity increased (P0.05), serum and liver M DA content decreased significantly (P0.05), liver inflammatory cytokines IL-1 尾 and IL-6 mRNA relative expression decreased significantly (P0.05). It can be seen that long-term feeding of Fusarium toxin can cause liver damage, affect liver antioxidant, protein synthesis and immune function in piglets, but after 21 days of recovery, Liver antioxidation and immune function of piglets were improved to some extent.
【作者单位】：山东农业大学动物科技学院;南京农业大学动物科技学院;
【基金】：山东省现代农业产业技术体系生猪创新团队建设项目(SDAIT-06-022-05)
【分类号】：S858.28

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