酵母浸出物对屎肠球菌活菌数的影响

发布时间：2019-01-05 06:55

【摘要】：屎肠球菌(Enterococcus faecium)发酵饲料中的活菌数是保证其功能特性的关键因素,为了提高屎肠球菌发酵过程中的活菌总数,本研究以屎肠球菌为试验菌株,选用4种不同特性的酵母浸出物来考察其对屎肠球菌活菌数的影响。通过单因素试验,确定以酵母浸出物为唯一氮源培养屎肠球菌的活菌数,根据酵母浸出物检测指标找出影响屎肠球菌活菌的关键因子。试验结果表明,培养基中主要提供生长因子和微量元素的酵母浸出物对屎肠球菌的活性有明显的影响,其中游离核苷酸的含量是影响屎肠球菌发酵活菌数重要因子,通过外源添加一定量的游离核苷酸,厌氧培养12 h,获得的最终活菌数为176×10~8 CFU/m L,与未添加游离核苷酸相比,活菌数提高了将近40%。结果显示,在培养基中添加一定量的游离核苷酸可提高屎肠球菌在发酵液中活菌数量,为工业化生产高活菌数的饲用屎肠球菌提供参考和借鉴。
[Abstract]:The number of live bacteria in the fermenting feed of Enterococcus faecium (Enterococcus faecium) is the key factor to ensure its functional characteristics. In order to increase the total number of live bacteria in the fermentation process of Enterococcus faecium, this study took Enterococcus faecium as the experimental strain. Four kinds of yeast extracts with different characteristics were used to study the effect of yeast extract on the number of live bacteria of Enterococcus faecium. According to the single factor test, the number of live bacteria of Enterococcus faecium cultured with yeast extract as the sole nitrogen source was determined, and the key factors affecting the living bacteria of Enterococcus faecium were found out according to the detection index of yeast extract. The results showed that the yeast extract, which mainly provided growth factors and trace elements, had a significant effect on the activity of Enterococcus faecium, and the content of free nucleotides was an important factor affecting the number of viable bacteria in fermentation of Enterococcus faecium. By adding a certain amount of free nucleotides and anaerobic culture for 12 h, the final viable bacteria number was 176 脳 10 ~ (8) CFU/m / L, which increased the number of live bacteria by nearly 40% compared with that without free nucleotides. The results showed that adding a certain amount of free nucleotides to the culture medium could increase the number of live bacteria of Enterococcus faecium in fermentation broth and provide a reference for the industrialized production of Enterococcus faecium with high number of live bacteria.
【作者单位】：中国热带农业科学院热带生物技术研究所;湖北海宜生物科技有限公司;江南大学生物工程学院;
【基金】：中国热带农业科学院基本科研业务费基金(1630052016007、1630052016012、1630052016021)
【分类号】：S816.3

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