鸭坦布苏病毒E蛋白线性化B细胞抗原表位的鉴定
发布时间：2019-01-09 06:32
【摘要】：为鉴定鸭坦布苏病毒(duck Tembusu virus,DTMUV)的B细胞抗原表位,以纯化的DTMUV YY5株为抗原制备了2株单克隆抗体AE4和BD10,研究证实BD10为线性化表位,其结合的抗原表位位于E蛋白的第三结构域(DomainⅢ,D_Ⅲ)。将DTMUV E蛋白D_Ⅲ结构域基因截短为具有末端相互重叠的15段,与MBP融合表达后以单克隆抗体BD10进行肽库扫描,结果筛选出DTMUV一个B细胞线性表位_(385)LVGSGKGQI_(393)(EP385)。该表位原核融合表达产物免疫小鼠制备的抗体,能够和DTMUV E蛋白反应,表明EP385表位具有良好的免疫原性,可为DTMUV多肽疫苗的研制及特异血清学诊断方法的开发奠定基础。
[Abstract]:In order to identify B cell epitopes of duck tambusuvirus (duck Tembusu virus,DTMUV), two monoclonal antibodies, AE4 and BD10, were prepared with purified DTMUV YY5 strain as antigen. The results showed that BD10 was a linearized epitope. The binding epitopes are located in the third domain of E protein (Domain 鈪，
本文编号：2405249
[Abstract]:In order to identify B cell epitopes of duck tambusuvirus (duck Tembusu virus,DTMUV), two monoclonal antibodies, AE4 and BD10, were prepared with purified DTMUV YY5 strain as antigen. The results showed that BD10 was a linearized epitope. The binding epitopes are located in the third domain of E protein (Domain 鈪，
本文编号：2405249
本文链接：https://www.wllwen.com/yixuelunwen/dongwuyixue/2405249.html
