布鲁氏菌病抗体不同检测方法的比较

发布时间：2019-01-09 10:05

【摘要】：布鲁氏菌病(简称布病)是一种危害养殖业的重要疫病。为了筛选出布病净化过程当中适合的检测方法,从而做好动物布病的诊断和净化工作,对GB/T 18646—2002和世界动物卫生组织(OIE)推荐的布鲁氏菌病抗体检测方法进行了比较。结果表明:在检测牛、羊血清布鲁氏菌病抗体的试验中,虎红平板凝集试验与其他方法差异最大,Kappa分析值(K值)在0.122~0.701之间,两种ELISA法一致性最高,K值在0.9以上,ELISA法和试管凝集试验、补体结合试验的K值在0.329~0.566之间,一致性较差,试管凝集试验和补体结合试验的K值在0.483~0.723之间,一致性较好。说明虎红平板凝集试验的敏感性最高,但是易出现假阳性,适合初步筛选,而在确诊时应优先选择间接ELISA试验或竞争ELISA试验,其次是试管凝集试验或补体结合试验。
[Abstract]:Brucellosis (brucellosis) is an important disease that endangers the breeding industry. In order to screen out suitable detection methods in the process of brucellosis purification and to do well in the diagnosis and purification of brucellosis, a comparison was made between GB/T 18646-2002 and brucellosis antibody detection method recommended by (OIE) of the World Organization for Animal Health. The results showed that in the test for detection of brucellosis antibody in cattle and sheep serum, Tiger red plate agglutination test had the greatest difference with other methods, the Kappa analysis value (K value) was between 0.122 and 0.701, and the two ELISA methods had the highest consistency. The K value of ELISA method and test tube agglutination test and complement binding test was 0.329566, the consistency was not good, and the K value of test tube agglutination test and complement binding test was 0.4830.723, the consistency was better than that of the test tube agglutination test and the complement binding test, and the K value of the test tube agglutination test and the test tube agglutination test was better. The results showed that the sensitivity of Tiger red plate agglutination test was the highest, but false positive was easy to appear, which was suitable for preliminary screening. Indirect ELISA test or competitive ELISA test should be selected first when diagnosis was confirmed, followed by test tube agglutination test or complement binding test.
【作者单位】：辽宁省动物疫病预防控制中心;辽宁省动物医学研究院;
【基金】：基金项目:辽宁省畜间布鲁氏菌病生态分布及防控策略研究(2014214016)
【分类号】：S855.12

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