鸡源产谷氨酸芽孢杆菌耐受稳定性研究

发布时间：2019-01-26 17:11

【摘要】：试验利用已有具有产谷氨酸的5株芽孢杆菌为试验菌株,研究其耐受稳定性,以利于该芽孢杆菌在动物饲料中添加应用。通过测定该5株芽孢杆菌第1代、第20代在不同p H值的酸和不同浓度胆盐的培养液中的生长情况,对试验芽孢杆菌进行耐受稳定性进行研究。结果显示,5株后备芽孢杆菌经过20代培养,表现出不同的耐受性。其中,第1代芽孢杆菌中J2、I3、C34在p H=1.5时的存活率为23.92%~46.43%;第20代芽孢杆菌中J2、I3、C34在p H=1.5时的存活率为16.41%~54.73%。在0.3%浓度的胆盐中,第1代芽孢杆菌中J2、I3、C34的存活率为54.07%~91.51%;在0.3%浓度的胆盐下,第20代芽孢杆菌中,J2、I3、C34的存活率为10.53%~92.25%。通过以上结果指出芽孢杆菌J2在不同p H值和胆盐浓度下稳定性最高,并具有传代稳定耐受性,可以作为微生态制剂后备菌深入研究。
[Abstract]:Five strains of Bacillus with glutamic acid production were used as experimental strains to study the stability of their tolerance in order to facilitate the application of Bacillus spp. in animal feed. The tolerance stability of the five strains of Bacillus spp. Was studied by measuring the growth of the first generation and the 20th generation of Bacillus in different pH values of acid and different concentrations of bile salt. The results showed that 5 strains of Bacillus reserve showed different tolerance after 20 generations of culture. Among them, the survival rate of J _ 2 I _ 3N _ 3C _ 34 at pH = 1.5 was 23.92 and 46.43 in the first generation of Bacillus sp., and the survival rate of J _ (2) I _ (3) C _ (34) in the 20th generation of Bacillus spp was 16.41% and 54.73% at pH = 1.5. In the bile salt of 0.3% concentration, the survival rate of J2I3C34 in the first generation of Bacillus spp. Was 54.07 and 91.51; In the 20 ~ (th) generation of Bacillus, the survival rate of J _ (2) I _ (3) C _ (34) was 10.53 ~ 92.25 under 0.3% concentration of bile salt. The results indicated that Bacillus sp. J _ 2 had the highest stability under different pH values and bile salt concentrations, and had subculture stability and tolerance, so it could be used as a back-up microorganism for further study.
【作者单位】：黑龙江八一农垦大学动物科技学院;辽宁省阜新市育才中学;
【基金】：黑龙江八一农垦大学大学生创新项目[XC2015023] 黑龙江省自然基金项目[C2015040,C201444]
【分类号】：S816

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