日粮烟酸水平对生长獭兔生产性能、血清抗氧化及脂肪代谢的影响
发布时间：2019-01-27 11:51
【摘要】：本课题研究了不同日粮烟酸添加水平对3-5月龄生长獭兔生产性能、血清抗氧化指标和脂肪代谢的影响。试验选用平均体重为(1860±144)g的3月龄健康獭兔160只,公母各半,按体重随机将其分成4组,每组40只,每个重复1只。饲喂4种烟酸添加水平不同的试验日粮(其中对照组为基础日粮,其他3组分别饲喂添加烟酸水平为50、100、200mg/kg的日粮),预饲7天,正式试验期53天。试验研究结果如下:日粮添加不同烟酸水平对日采食量、日增重、料重比均无显著影响(P0.05);日粮烟酸水平对毛皮重量、毛皮面积和被毛长度没有显著影响(P0.05),对毛皮厚度影响显著(P=0.0459),对毛囊密度、初级毛囊密度、次级毛囊密度、初级毛囊密度/次级毛囊密度影响极显著(P0.05),2组和3组的密度极显著高于1组和4组;日粮烟酸水平对獭兔肉质的亮度、红度、pH24、滴水损失及压力损失没有显著影响(P0.05),对肉质黄度的影响极显著(P=0.0014),3组的黄度值极显著低于其它组;随着日粮烟酸水平增加,血清谷胱甘肽过氧化氢酶、超氧化物歧化酶、丙二醛含量变化差异不显著(P0.05);日粮烟酸水平对血清载脂蛋白A、极低密度脂蛋白、低密度脂蛋白、总蛋白含量影响不显著(P0.05),对载脂蛋白B(P=0.0023)、高密度脂蛋白(P=0.0002)含量影响极显著;日粮烟酸水平对血浆甘油三酯、雌二醇、瘦素、胰岛素含量影响不显著(P0.05),对总胆固醇(P0.0001)和游离脂肪酸(P0.0001)含量影响极显著,随着日粮烟酸水平的增加,游离脂肪酸呈极显著下降趋势,对脂联素(P=0.0324)含量影响显著;日粮烟酸水平对肝脏载脂蛋白A、总蛋白含量影响不显著(P0.05),对肝脏载脂蛋白B含量影响极显著(P=0.0002);日粮烟酸水平对肝脏苹果酸脱氢酶活性影响不显著(P0.05),对血浆脂蛋白酯酶活性影响极显著(P0.0001),对血浆苹果酸脱氢酶(P=0.0210)活性和肝脏脂肪酸合成酶(P=0.0428)活性影响显著;随着日粮烟酸水平的增加,脂联素受体1、胰岛素受体、肉碱脂酰转移酶基因表达量没有显著变化(P0.05),脂联素受体2基因表达量显著降低(P=0.0129),乙酰辅酶A羧化酶基因表达量极显著降低(P=0.0011),表明添加烟酸能够显著抑制脂联素受体2和乙酰辅酶A羧化酶基因的转录水平。
[Abstract]:The effects of different dietary niacin supplementation levels on the production performance, serum antioxidant index and fat metabolism of 3-5 month old growing Rex rabbits were studied. 1860 卤144g healthy rex rabbits with average weight of (1860 卤14.4) g, male and female, were randomly divided into 4 groups according to their body weight, 40 rabbits in each group, with one repeats in each group. Four experimental diets with different levels of nicotinic acid were fed (the control group was the basal diet and the other three groups were fed the diet supplemented with nicotinic acid level of 50100200mg/kg) for 7 days and the formal trial period was 53 days. The results were as follows: dietary niacin supplementation had no significant effect on daily intake, daily gain and feed weight ratio (P0.05); Dietary niacin level had no significant effect on fur weight, fur area and coat length (P0.05), but had significant effect on fur thickness (P0. 0459), hair follicle density, primary hair follicle density and secondary hair follicle density. The primary hair follicle density / secondary hair follicle density was significantly affected (P0.05), the density of group 2 and group 3 was significantly higher than that of group 1 and group 4. Dietary niacin level had no significant effect on meat brightness, redness, pH24, drop loss and pressure loss (P0.05), but had very significant effect on meat yellowness (P0. 0014). The yellow value of three groups was significantly lower than that of other groups. With the increase of niacin level, there was no significant difference in serum glutathione catalase, superoxide dismutase and malondialdehyde (P0.05). Dietary niacin level had no significant effect on serum apolipoprotein A, very low density lipoprotein and total protein content (P0.05), but had very significant effect on apolipoprotein B (P0. 0023) and high density lipoprotein (P0. 0002) content. Dietary niacin levels had no significant effect on plasma triglyceride, estradiol, leptin and insulin contents (P0.05), but had significant effects on total cholesterol (P0.0001) and free fatty acids (P0.0001). With the increase of niacin level in diet, the free fatty acid decreased significantly, and the content of adiponectin (P0. 0324) was significantly affected. Dietary nicotinic acid level had no significant effect on liver apolipoprotein A and total protein content (P0.05), but had extremely significant effect on liver apolipoprotein B content (P0. 0002). Dietary niacin level had no significant effect on hepatic malate dehydrogenase activity (P0.05), but had extremely significant effect on plasma lipoprotein esterase activity (P0.0001). The activity of plasma malate dehydrogenase (Pt0. 0210) and hepatic fatty acid synthase (P0. 0428) were significantly affected. With the increase of dietary nicotinic acid level, adiponectin receptor 1, insulin receptor, carnitine adiponectin receptor 2 gene expression did not change significantly (P0.05), adiponectin receptor 2 gene expression decreased significantly (P0. 0129). The expression of acetyl coA carboxylase gene was significantly decreased (P0. 0011), indicating that nicotinic acid could significantly inhibit the transcription level of adiponectin receptor 2 and acetyl coA carboxylase gene.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S829.1
本文编号：2416233
[Abstract]:The effects of different dietary niacin supplementation levels on the production performance, serum antioxidant index and fat metabolism of 3-5 month old growing Rex rabbits were studied. 1860 卤144g healthy rex rabbits with average weight of (1860 卤14.4) g, male and female, were randomly divided into 4 groups according to their body weight, 40 rabbits in each group, with one repeats in each group. Four experimental diets with different levels of nicotinic acid were fed (the control group was the basal diet and the other three groups were fed the diet supplemented with nicotinic acid level of 50100200mg/kg) for 7 days and the formal trial period was 53 days. The results were as follows: dietary niacin supplementation had no significant effect on daily intake, daily gain and feed weight ratio (P0.05); Dietary niacin level had no significant effect on fur weight, fur area and coat length (P0.05), but had significant effect on fur thickness (P0. 0459), hair follicle density, primary hair follicle density and secondary hair follicle density. The primary hair follicle density / secondary hair follicle density was significantly affected (P0.05), the density of group 2 and group 3 was significantly higher than that of group 1 and group 4. Dietary niacin level had no significant effect on meat brightness, redness, pH24, drop loss and pressure loss (P0.05), but had very significant effect on meat yellowness (P0. 0014). The yellow value of three groups was significantly lower than that of other groups. With the increase of niacin level, there was no significant difference in serum glutathione catalase, superoxide dismutase and malondialdehyde (P0.05). Dietary niacin level had no significant effect on serum apolipoprotein A, very low density lipoprotein and total protein content (P0.05), but had very significant effect on apolipoprotein B (P0. 0023) and high density lipoprotein (P0. 0002) content. Dietary niacin levels had no significant effect on plasma triglyceride, estradiol, leptin and insulin contents (P0.05), but had significant effects on total cholesterol (P0.0001) and free fatty acids (P0.0001). With the increase of niacin level in diet, the free fatty acid decreased significantly, and the content of adiponectin (P0. 0324) was significantly affected. Dietary nicotinic acid level had no significant effect on liver apolipoprotein A and total protein content (P0.05), but had extremely significant effect on liver apolipoprotein B content (P0. 0002). Dietary niacin level had no significant effect on hepatic malate dehydrogenase activity (P0.05), but had extremely significant effect on plasma lipoprotein esterase activity (P0.0001). The activity of plasma malate dehydrogenase (Pt0. 0210) and hepatic fatty acid synthase (P0. 0428) were significantly affected. With the increase of dietary nicotinic acid level, adiponectin receptor 1, insulin receptor, carnitine adiponectin receptor 2 gene expression did not change significantly (P0.05), adiponectin receptor 2 gene expression decreased significantly (P0. 0129). The expression of acetyl coA carboxylase gene was significantly decreased (P0. 0011), indicating that nicotinic acid could significantly inhibit the transcription level of adiponectin receptor 2 and acetyl coA carboxylase gene.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S829.1
【参考文献】
相关期刊论文 前1条
1 吴妙宗,蔡辉益;烟酸的作用与需要量[J];中国饲料;2001年14期
，本文编号：2416233
本文链接：https://www.wllwen.com/yixuelunwen/dongwuyixue/2416233.html
