促卵泡素和促黄体生成素对儋州鸡生殖激素调控规律的研究

发布时间：2019-02-09 13:15

【摘要】：为研究促卵泡素(FSH)和促黄体生成素(LH)对儋州鸡体内其他生殖激素的调控规律,本试验通过改变FSH和LH在儋州鸡血液中的浓度,并采用双抗体一步夹心法酶联免疫吸附试验(ELISA)对处理前后儋州鸡血液中FSH、LH、催乳素(PRL)、孕酮(P)、雌二醇(E2)的浓度进行测定。结果发现,注射外源性FSH和LH分别能提高儋州鸡血液中FSH和LH浓度;当儋州鸡血液中FSH或LH浓度显著升高时则均能引起PRL浓度显著降低(P0.05),但当FSH和LH浓度同时显著升高时,PRL浓度显著升高(P0.05);当儋州鸡血液中FSH浓度显著升高时,E2及P浓度显著提升(P0.05),且在高浓度LH的协同下提升幅度更大;当儋州鸡血液中LH浓度显著升高时E2及P浓度升高但不显著(P0.05)。本研究结果表明,儋州鸡血液中FSH或LH浓度的提高均能降低PRL的浓度,并能不同程度的提升E2及P的浓度,但FSH与LH浓度同时提高则能通过协同作用刺激E2及P浓度的大幅提升,当E2及P浓度过高时能通过刺激PRL的释放,负反馈调节血液中FSH与LH,并恢复血液中E2及P浓度。
[Abstract]:In order to study the regulation of follicle stimulating hormone (FSH) and luteinizing hormone (LH) on other reproductive hormones in Danzhou chicken, the concentration of FSH and LH in the blood of Danzhou chicken was changed. The concentration of FSH,LH, prolactin (PRL), progesterone (P), estradiol (E2) in Danzhou chicken blood was determined by double antibody one step sandwich enzyme-linked immunosorbent assay (ELISA) before and after treatment. The results showed that injection of exogenous FSH and LH could increase the concentration of FSH and LH in Danzhou chicken blood, respectively. When the concentration of FSH or LH in the blood of Danzhou chicken increased significantly, the concentration of PRL decreased significantly (P0.05), but when the concentration of FSH and LH increased significantly, the concentration of PRL increased significantly (P0.05). When the concentration of FSH in blood of Danzhou chicken increased significantly, the concentration of E _ 2 and P increased significantly (P0.05), and the increase of E _ 2 and P was greater under the coordination of high concentration of LH. When the concentration of LH in blood of Danzhou chicken increased significantly, the concentration of E2 and P increased but not significantly (P0.05). The results showed that the increase of FSH or LH in blood of Danzhou chicken could decrease the concentration of PRL and increase the concentration of E2 and P in different degree. However, increasing the concentration of FSH and LH at the same time could stimulate the increase of E _ 2 and P concentration through synergistic effect. When the concentration of E _ 2 and P was too high, it could stimulate the release of PRL, regulate FSH and LH, in blood and restore the E _ 2 and P concentrations in blood by negative feedback.
【作者单位】：海南大学热带农林学院;中国热带农业科学院热带作物品种资源研究所;
【基金】：儋州小种肉鸡生产性能测定和选育研究(HD-KYH-2015070)
【分类号】：S831

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