三个地方鸡种部分组织脂肪代谢差异的遗传因素研究

发布时间：2019-02-09 14:25

【摘要】：本实验以长顺绿壳蛋鸡、兴义矮脚黄鸡和隐性白羽鸡三个不同的贵州本地品种为研究对象,通过对不同品种、不同生长阶段雌雄个体的屠宰性状指标,结合各个不同个体的心脏、肝脏、腿肌、胸肌、皮下脂肪及腹部脂肪六个组织中FASN基因和ACC基因的表达含量分析,及各个品种的FASN基因和ACC基因启动子区的生物信息学信息,探索FASN、ACC两个不同的基因与脂肪沉积效应之间的生物信息学关系,以期找到影响脂肪沉积代谢差异的遗传学因素。研究结果如下:1.绿壳蛋鸡的腹部脂肪沉积率明显低于兴义矮脚黄鸡和隐性白羽鸡的腹脂沉积率,差异极显著(P0.01)。2.绿壳蛋鸡的胸肌和腿肌的肌内脂肪含量明显低于兴义矮脚黄鸡和隐性白羽鸡的胸肌和腿肌的肌内脂肪含量,差异极显著(P0.01)。3.FASN基因的启动子区含有7个不同的SNP位点,且这7个SNP位点在FASN基因的表达上未产生明显的影响作用。FASN基因启动子区序列不是本实验所研究的三个品种产生脂肪沉积差异的遗传因素。4.FASN基因和ACC基因在不同个体不同组织中的表达存在明显的差异性。品种特异性为:相同生长阶段的绿壳蛋鸡不同组织的基因表达含量低于兴义矮脚黄鸡和隐性白羽鸡的表达含量;组织特异性为:腹部脂肪表达量皮下脂肪肝脏鸡肉组织。5.FASN基因和ACC基因的表达量与腹部脂肪沉积量存在正相关性关系。表明,FASN基因和ACC基因是调控脂肪沉积代谢的主要遗传因素。
[Abstract]:In this experiment, three different Guizhou native breeds, Changshun green shell laying hens, Xingyi dwarf yellow chickens and recessive white feather chickens, were used to study the slaughter characteristics of female and male individuals in different breeds and different growth stages. The expression of FASN gene and ACC gene in heart, liver, leg muscle, pectoralis muscle, subcutaneous fat and abdominal fat were analyzed, and the bioinformatics information of FASN gene and ACC gene promoter region were analyzed. To explore the bioinformatics relationship between two different genes of FASN,ACC and lipid deposition effect in order to find out the genetic factors that influence the difference of fat deposition metabolism. The results are as follows: 1. The abdominal fat deposition rate of green shell laying chicken was significantly lower than that of Xingyi dwarf yellow chicken and recessive white feather chicken (P0.01). The contents of intramuscular fat in the pectoral and leg muscles of the green shell laying hens were significantly lower than those in the pectoral and leg muscles of Xingyi dwarf yellow chickens and recessive white feather chickens. The difference was very significant (P0.01). There were 7 different SNP loci in the promoter region of 3.FASN gene. The seven SNP loci had no significant effect on the expression of FASN gene. The promoter sequence of FASN gene was not the genetic factor of fat deposition difference among the three cultivars studied in this study. 4.FASN gene and ACC gene There are obvious differences in expression in different tissues and individuals. The variety specificity was: the gene expression content in different tissues of green shell layer at the same growth stage was lower than that of Xingyi dwarf yellow chicken and recessive white feather chicken; The expression of 5.FASN gene and ACC gene were positively correlated with abdominal fat deposition. The results showed that FASN gene and ACC gene were the main genetic factors regulating fat deposition metabolism.
【学位授予单位】：贵州大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S831

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