产复合酶优良菌株筛选及苹果渣固态发酵效果研究

发布时间：2019-02-13 04:12

【摘要】：通过液态发酵产酶试验和苹果渣固态发酵试验,研究不同丝状真菌产水解酶的活性及产酶优良的霉菌对苹果渣发酵的改质效果,筛选出适用于苹果渣发酵的优良霉菌。结果表明,黑曲霉MHQ1产纤维素酶、果胶酶和木聚糖酶的活性较高,分别为64.7、66.9和216.7U·mL~(-1);米曲霉MMQ1产淀粉酶和蛋白酶的活性较高,分别为1 782.6和83.0U·mL~(-1);烟曲霉MYQ1产蛋白酶和果胶酶的活性较高,分别为128.9和51.6U·mL~(-1)。其中,黑曲霉MHQ1能在果渣基质上良好地生长,对果渣具有较强的降解改质能力,产物中粗蛋白和纯蛋白质量分数分别增至387.5和228.9g·kg~(-1),接菌增率为79.2%和193.8%,且纤维素酶、果胶酶和蛋白酶的活性分别为178.0、150.6和57.5U·g~(-1),纤维素、果胶分别降解28.9%、53.8%,水溶性氨基酸质量分数增至2.7%。采用酵母菌与产复合酶优良的霉菌复合发酵,能显著提高果渣发酵产物中的蛋白质水平,降低抗营养物质的质量分数,提高水解酶和水溶性氨基酸等活性物质的质量分数,有效地发酵果渣为生物蛋白饲料。
[Abstract]:The activity of hydrolase produced by different filamentous fungi and the modification effect of fine fungi on the fermentation of apple pomace were studied by liquid fermentation and solid state fermentation of apple pomace. The results showed that the activities of Cellulase, Pectinase and Xylanase from Aspergillus Niger were 64.7U mL~ (-1) and 216.7U mL~ (-1), respectively. The activities of amylase and protease produced by Aspergillus oryzae MMQ1 were 1 782.6 U mL~ (-1) and 83.0 U mL~ (-1), respectively, and the activities of MYQ1 producing protease and Pectinase from Aspergillus fumigatus were higher (128.9 U mL~ (-1) and 51.6 U mL~ (-1) respectively). Among them, Aspergillus Niger MHQ1 can grow well on the fruit residue substrate, and has a strong ability to degrade and improve the fruit residue. The content of crude protein and pure protein in the product increased to 387.5 and 228.9 g kg~ (-1), respectively, and the content of crude protein and pure protein in the product increased to 387.5 and 228.9 g kg~ (-1), respectively. The inoculation rate was 79.2% and 193.8%, and the activities of cellulase, Pectinase and protease were 178.0150.6 and 57.5 U g-1, respectively. The mass fraction of water-soluble amino acids increased to 2.7. The protein level in the fermentation product of fruit residue was significantly increased, the mass fraction of anti-nutritive substance was decreased, and the mass fraction of hydrolase and water-soluble amino acid were increased by using the compound fermentation of yeast and mold with excellent compound enzyme. Effectively fermented fruit residue is bioprotein feed.
【作者单位】：西北农林科技大学资源环境学院;
【基金】：“十二五”国家科技支撑计划(2012BAD14B11)~~
【分类号】：S816.6

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