猪肺炎支原体与猪鼻支原体分离鉴定及鉴别检测

发布时间：2019-02-15 12:44

【摘要】：为建立猪肺炎支原体与猪鼻支原体的鉴别诊断方法,对从河南省及周边地区猪场采集的患呼吸道疾病的猪只样品进行支原体的分离培养,针对猪肺炎支原体与猪鼻支原体设计了2对引物,扩增片段大小分别为480 bp和360 bp,对所分离纯化的病原微生物进行了菌落形态观察及分子鉴定。结果显示,分离的病原微生物为支原体,菌落呈典型的"煎蛋样"特征;通过单一PCR扩增反应,分别得到了猪肺炎支原体与猪鼻支原体相应的目的扩增片段。表明分离到了猪肺炎支原体与猪鼻支原体。在单一扩增的基础上,建立了猪肺炎支原体与猪鼻支原体二重PCR反应方法,可同时得到2条目的条带,与单一PCR扩增结果一致。表明建立的二重PCR方法可用于猪肺炎支原体与猪鼻支原体的同时检测和鉴别诊断。
[Abstract]:In order to establish a method for differential diagnosis of mycoplasma pneumoniae and mycoplasma isolated from pigs with respiratory diseases collected from pig farms in Henan province and its surrounding areas. Two pairs of primers were designed for mycoplasma pneumoniae and mycoplasma suis. The amplified fragments were 480 bp and 360 bp, respectively. The colony morphology and molecular identification of the isolated and purified pathogenic microorganisms were studied. The results showed that the isolated pathogenic microorganism was mycoplasma and the colony had the characteristic of "omeloid", and the corresponding amplified fragments of Mycoplasma pneumoniae and Mycoplasma suis were obtained by a single PCR amplification reaction. The results showed that mycoplasma pneumoniae and mycoplasma suis were isolated. On the basis of single amplification, a double PCR reaction method of mycoplasma pneumoniae and mycoplasma suis was established. The bands of two items were obtained simultaneously, which were consistent with the results of single PCR amplification. This method can be used for simultaneous detection and differential diagnosis of mycoplasma pneumoniae and mycoplasma suis.
【作者单位】：河南省农业科学院畜牧兽医研究所;郑州幼儿师范高等专科学校;
【基金】：河南省农业科学院自主创新基金项目(2016ZC49)
【分类号】：S858.28

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