致林麝猝死的病原豚鼠气单胞菌的分离与鉴定

发布时间：2019-02-22 20:41

【摘要】：为了确定引起两头圈养林麝猝死的主要病原菌,确定合理的临床用药和预防方案,本研究采用血平板对死亡林麝的心、肝、脾、肺、肾等组织器官中可疑病原进行分离,通过菌落形态学观察、革兰染色镜检、16S rDNA序列测定分析、生化试验、致病性试验及药敏试验(DST)进行鉴定。通过16S rDNA基因的系统进化研究发现,从猝死林麝体内分离的细菌与豚鼠气单胞菌S5-3株的同源性高达99.9%,结合生化试验结果进行综合分析,确定致林麝猝死的致病菌为豚鼠气单胞菌。药敏试验结果显示,该菌株对氨苄西林、卡那霉素、链霉素、环丙沙星、头孢哌酮较敏感,对头孢他啶、哌拉西林、庆大霉素、复方新诺明、四环素为中敏,对红霉素、青霉素、利福平、头孢拉定、呋喃妥因存在多重耐药性;通过PCR检测分析,发现该菌株携带耐药基因bla-OXA和bla-PER;动物试验表明,给予小鼠接种3.9×10~8 CFU/0.3 m L的剂量会导致小鼠全部死亡,说明该菌株具有较强致病性。本研究结果对林麝养殖生产中临床用药具有一定的指导意义。
[Abstract]:In order to determine the main pathogenic bacteria causing sudden death in two captive forest musk deer, and to determine the rational clinical medication and prevention plan, this study used blood plate to isolate suspicious pathogens in the heart, liver, spleen, lung, kidney and other tissues and organs of the dead forest musk deer. Colony morphology, Gram staining, 16s rDNA sequence analysis, biochemical test, pathogenicity test and drug sensitivity test (DST) were used for identification. The phylogenetic study of 16s rDNA gene showed that the homology of bacteria isolated from sudden death forest musk deer and Aeromonas guinea-pig S5-3 strain was 99.9%. The pathogen causing sudden death of musk deer was Aeromonas guinea-pig. The results of drug sensitivity test showed that the strain was sensitive to ampicillin, kanamycin, streptomycin, ciprofloxacin, cefoperazone, ceftazidime, piperacillin, gentamicin, compound sulfamide, tetracycline as medium sensitivity and erythromycin, Penicillin, rifampicin, cefradine, furantoin have multidrug resistance; The results of PCR analysis showed that the strain carrying the drug resistance gene bla-OXA and bla-PER; showed that the dose of 3.9 脳 10 8 CFU/0.3 mL could lead to the death of all the mice, which indicated that the strain had strong pathogenicity. The results of this study have a certain guiding significance for the clinical use of musk deer in the production of forest musk deer.
【作者单位】：四川农业大学动物医学院;四川夹金山逢春养殖科技有限公司;
【基金】：四川省科技支撑计划项目(2014SZ0132)
【分类号】：S858.94

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