Bta-miR-202靶向SEPT7调控牛核移植胚胎第一次卵裂的研究
发布时间：2019-02-24 11:18
【摘要】：MicroRNA在胚胎合子基因转换、胚胎致密化、DNA甲基化和染色质重塑等方面发挥重要作用;精子中的microRNA可通过调控早期胚胎转录水平,直接参于胚胎的发育,并在跨代遗传中发挥重要作用。相比体外受精胚胎,体细胞核移植胚胎表现出较低的发育潜能。目前多认为这是技术层面不足和异常重编程导致的,而精子携带进入卵母细胞的RNA的作用一直被忽视。本试验以精源性microRNA为切入点,研究了其对早期核移植胚胎发育的影响。主要研究结果如下:Bta-miR-202在精子中高表达,并且在受精胚胎原核时期的表达水平高于卵母细胞以及2细胞时期的受精胚胎。本课题组前期研究证明SEPT7是bta-miR-202的一个靶基因,生物信息学方法分析显示SEPT7在细胞胞质分裂中发挥重要作用;对SEPT7进行蛋白互作分析,结果提示SEPT7可能与HDAC6相互作用。SEPT7蛋白在类原核期核移植胚胎的表达水平高于原核期体外受精胚;获得bta-miR-202核移植胚胎的SEPT7表达水平低于对照组,但仍高于体外受精组;获得bta-miR-202核移植胚胎的α-tubulin乙酰化水平高于对照组,但仍低于体外受精组;获得bta-miR-202核移植胚胎第一次卵裂发生碎片率低于对照组,但仍高于体外受精组。Bta-miR-202靶向卵源SEPT7,可能通过SEPT7与HDAC6的互作,调控a-tubulin乙酰化水平从而影响核移植胚胎的第一次卵裂。综上所述,bta-miR-202靶向SEPT7调控牛核移植胚胎第一次卵裂。
[Abstract]:MicroRNA plays an important role in embryo zygote gene transformation, embryo densification, DNA methylation and chromatin remodeling. MicroRNA in spermatozoa can be directly involved in the development of embryos by regulating the transcriptional level of early embryos and play an important role in cross-generation inheritance. Compared with IVF embryos, somatic nuclear transfer embryos showed lower developmental potential. At present, it is believed that this is caused by inadequate technology and abnormal reprogramming, while the role of sperm carrying RNA into oocytes has been neglected. The effects of spermatogenic microRNA on the development of early nuclear transfer embryos were studied. The main results were as follows: the expression of Bta-miR-202 was higher in spermatozoa than in oocytes and 2-cell embryos. Our previous studies have proved that SEPT7 is a target gene of bta-miR-202. Bioinformatics analysis shows that SEPT7 plays an important role in cytokinesis. The results of protein interaction analysis of SEPT7 suggest that SEPT7 may interact with HDAC6. The expression level of SEPT7 protein in prokaryotic phase nuclear transfer embryos is higher than that in prokaryote phase in vitro fertilization embryos. The expression of SEPT7 in the bta-miR-202 nuclear transfer embryos was lower than that in the control group, but still higher than that in the IVF group, and the level of 伪-tubulin acetylation in the bta-miR-202 nuclear transfer embryos was higher than that in the control group, but still lower than that in the IVF group. The first cleavage rate of bta-miR-202 nuclear transfer embryos was lower than that of control group, but still higher than that of IVF group. Bta-miR-202 targeted SEPT7, might interact with HDAC6 through SEPT7. Regulation of a-tubulin acetylation level affects the first cleavage of nuclear transfer embryos. In conclusion, bta-miR-202 targeting SEPT7 regulates the first cleavage of bovine nuclear transfer embryos.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S823
本文编号：2429503
[Abstract]:MicroRNA plays an important role in embryo zygote gene transformation, embryo densification, DNA methylation and chromatin remodeling. MicroRNA in spermatozoa can be directly involved in the development of embryos by regulating the transcriptional level of early embryos and play an important role in cross-generation inheritance. Compared with IVF embryos, somatic nuclear transfer embryos showed lower developmental potential. At present, it is believed that this is caused by inadequate technology and abnormal reprogramming, while the role of sperm carrying RNA into oocytes has been neglected. The effects of spermatogenic microRNA on the development of early nuclear transfer embryos were studied. The main results were as follows: the expression of Bta-miR-202 was higher in spermatozoa than in oocytes and 2-cell embryos. Our previous studies have proved that SEPT7 is a target gene of bta-miR-202. Bioinformatics analysis shows that SEPT7 plays an important role in cytokinesis. The results of protein interaction analysis of SEPT7 suggest that SEPT7 may interact with HDAC6. The expression level of SEPT7 protein in prokaryotic phase nuclear transfer embryos is higher than that in prokaryote phase in vitro fertilization embryos. The expression of SEPT7 in the bta-miR-202 nuclear transfer embryos was lower than that in the control group, but still higher than that in the IVF group, and the level of 伪-tubulin acetylation in the bta-miR-202 nuclear transfer embryos was higher than that in the control group, but still lower than that in the IVF group. The first cleavage rate of bta-miR-202 nuclear transfer embryos was lower than that of control group, but still higher than that of IVF group. Bta-miR-202 targeted SEPT7, might interact with HDAC6 through SEPT7. Regulation of a-tubulin acetylation level affects the first cleavage of nuclear transfer embryos. In conclusion, bta-miR-202 targeting SEPT7 regulates the first cleavage of bovine nuclear transfer embryos.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S823
【参考文献】
相关期刊论文 前1条
1 ;Nuclear reprogramming: the strategy used in normal development is also used in somatic cell nuclear transfer and parthenogenesis[J];Cell Research;2007年02期
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