务川黑牛遗传资源状况及保护对策

发布时间：2019-02-26 10:14

【摘要】：本文目的在于研究务川黑牛遗传资源状况及保护对策方面的相关信息。具体方法就是能够以以贵州地方牛优良品种务川黑牛为研究对象,选取分布于其7条染色体上的ETH10、ILSTS033、CSSM66、BM2113、ETH225、BM1818和CSRM60共7对微卫星引物进行遗传多样性分析;同时,基于务川黑牛的历史、发展及现状为基础,分析实际中务川黑牛在遗传资源保护方面具体的状况,然后可以通过优化建立基于务川黑牛的保护区、黑牛核心保种群的策略,以及对务川黑牛遗传资源进行开发性的保护,以确保务川黑牛遗传资源得到有效保护。结论证实,7个位点的平均等位基因数、平均有效等位基因数、平均期望杂合度、平均多态信息含量分别为3±1.15、2±0.89、0.6±0.10、0.47±0.13,说明务川黑牛具有良好的遗传多样性;通过格控制务川黑牛杂交代数,确保务川黑牛级进杂交不可以超过2代,也能够及时在务川黑牛杂种后代中,优化选择遗传性能优良的务川黑牛进行遗传横交固定,从而可以确保建立优良的务川黑牛品系,可以优化保护务川黑牛遗传资源,取得实效。结论表明,综合分析务川黑牛遗传资源保护方面的具体状况,并制定具体的务川黑牛遗传资源保护技术路线与对策,能够为今后务川黑牛遗传资源保护提供依据,发挥积极影响。
[Abstract]:The purpose of this paper is to study the status of genetic resources and protection strategies of Wuchuan black cattle. The specific method was to select 7 pairs of microsatellite primers (ETH10,ILSTS033,CSSM66,BM2113,ETH225,BM1818 and CSRM60) distributed on 7 chromosomes of Wuchuan Black cattle, an excellent breed of Guizhou local cattle, for genetic diversity analysis. At the same time, based on the history, development and present situation of Wuchuan black cattle, this paper analyzes the specific situation of the conservation of genetic resources in the actual Wuchuan black cattle, and then optimizes the establishment of the reserve based on the Wuchuan black cattle. The core conservation strategy of black cattle and the exploitation protection of genetic resources of Wuchuan black cattle were carried out in order to ensure the effective protection of the genetic resources of Wuchuan black cattle. Conclusion the average allele number, effective allele number, average expected heterozygosity and average polymorphism information content of 7 loci were 3 卤1.15, 2 卤0.89, 0.6 卤0.10,0.47 卤0.13, respectively, and the mean heterozygosity was 3 卤1.15, 2 卤0.89, 0.6 卤0.10, 0.47 卤0.13, respectively. The results indicated that Wuchuan Black cattle had good genetic diversity. By controlling the crossing algebra of Wuchuan black cattle, we can ensure that Wuchuan black cattle can not exceed 2 generations, and in time, we can optimize and select Wuchuan black cattle with good genetic performance for genetic cross-crossing and fixation in the hybrid offspring of Wuchuan black cattle. Therefore, it can ensure the establishment of excellent lines of Wuchuan black cattle, optimize the protection of genetic resources of Wuchuan black cattle, and achieve practical results. The conclusion shows that the comprehensive analysis of the specific situation of the conservation of the genetic resources of Wuchuan black cattle and the formulation of specific technical routes and countermeasures for the conservation of the genetic resources of Wuchuan black cattle can provide the basis for the protection of the genetic resources of Wuchuan black cattle in the future. Give full play to the positive influence.
【作者单位】：遵义职业技术学院;贵州省遵义市农业委员会;
【基金】：贵州省农业动植物育种专项,黔农育专字(2014)11号贵州省科技厅农业攻关计划,黔科合(2014)3049号
【分类号】：S823

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