皖北地区禽源大肠杆菌的血清型鉴定与耐药性分析

发布时间：2019-03-02 07:58

【摘要】：为了掌握皖北地区家禽致病性大肠杆菌的优势血清型及耐药性表现,从皖北4个市8个县区32个规模化养殖场采集疑似家禽大肠杆菌病的病料64份,通过常规分离培养纯化获取31株致病性大肠杆菌,对31株致病性大肠杆菌进行血清学鉴定和耐药性分析。试验结果表明:31株致病性大肠杆菌分别属于O_(78)、O_(18)、O_(88)、O_2、O_1、O_(143)、O_4、O_(35)、和O_(141)血清型,其中O_(78)9株、O_(18)7株、O_(88)5株,共计21株,占分离株的67.74%,属于本地区的优势血清型。致病性试验结果表明,所分离的31株致病菌中,83.9%具有高致病性,12.9%具有中致病性,3.2%具有低致病性,不同血清型致病性没有明显差异。分离菌株对11种抗生素表现不同程度的耐药性,对复方磺胺甲基异VA唑、磺胺二甲嘧啶、恩诺沙星表现严重耐药,耐药比例在70%以上;对阿莫西林、链霉素、庆大霉素、卡那霉素、氟苯尼考、环丙沙星耐药比例在16%以上;对头孢噻肟、头孢唑肟、阿米卞星耐药性比例在10%以下。其中,对4、5、6种药物表现耐药比例较高,占分离株的60%以上,对3、7、8、9耐药性居中,所有菌株均表现为多重耐药性。研究结果为皖北地区家禽大肠杆菌病的疫苗防控、药物防治提供了科学依据。
[Abstract]:In order to master the dominant serotype and drug resistance of pathogenic Escherichia coli in poultry in northern Anhui, 64 samples of suspected poultry Escherichia coli were collected from 32 large-scale farms in 4 cities and 8 counties in northern Anhui. 31 strains of pathogenic Escherichia coli were obtained by routine isolation, culture and purification. The serological identification and drug resistance analysis of 31 strains of pathogenic Escherichia coli were carried out. The results showed that 31 strains of pathogenic Escherichia coli belonged to serotypes O78, O18, O88, O2, O1, O143, O14, O35, and O141, respectively, and the serotypes were O78, O18, O88, O2, O1, O143, O4, O35 and O141, respectively. Among them, 9 strains were O78, 7 O18 and 5 O88, which accounted for 67.74% of the isolates, belonging to the dominant serotypes in this area. The results of pathogenicity test showed that 83.9% of the 31 isolates were highly pathogenic, 12.9% had moderate pathogenicity and 3.2% had low pathogenicity. There was no significant difference in pathogenicity among different serotypes. The isolates showed different drug resistance to 11 kinds of antibiotics, and showed serious resistance to compound sulfamethoxazole, sulfamethazine and enrofloxacin, the rate of drug resistance was more than 70%. The resistance to amoxicillin, streptomycin, gentamicin, kanamycin, flufenicol, ciprofloxacin and cefotaxime, cefotaxime and amibianin were more than 16% and less than 10%, respectively. Among them, to 4,5, 6 kinds of drugs showed high drug resistance rate, accounting for more than 60% of isolates, to 3,7,8,9 drug resistance was in the middle, all of the strains showed multi-drug resistance. 3, 7, 8, 9 strains showed multiple drug resistance. 3, 7, 8, 9 strains showed multiple resistance. The results provide scientific basis for vaccine control and drug control of poultry colibacillosis in northern Anhui.
【作者单位】：宿州职业技术学院;南京农业大学动物医学院;江苏省动物重要疫病与人兽共患病防控协同创新中心;
【基金】：安徽省自然科学基金(KJ2013B2950)
【分类号】：S852.61

【相似文献】