围产期奶牛专用微生态制剂的研制

发布时间：2019-03-03 12:33

【摘要】：目的根据反刍动物主要是依靠瘤胃发酵这一特点,研制出以丙酸菌为主的高效安全的奶牛瘤胃复合微生态制剂,可以提高瘤胃丙酸产生量,改善奶牛能量负平衡,从而解决生产中目前所面对的技术难题。方法通过健康奶牛瘤胃高产丙酸菌株的分离、鉴定,筛选瘤胃高产丙酸菌株和饲用酵母菌优势组合,制备高效、无污染的以丙酸高产菌为核心技术的围产期奶牛瘤胃微生态制剂。结果1、通过对瘤胃液中厌氧菌的分离成功分离出了丙酸生成菌,用生化特性和16s r RNA克隆的方法成功鉴定了高产丙酸生成菌埃氏巨型球菌、反刍月形单胞菌和小韦荣球菌。2、筛选了高产丙酸生成菌株和饲用酵母菌的最佳组合,即热带假丝酵母、酿酒酵母和产朊假丝酵母,以及三株丙酸生成菌,月形单胞菌菌液、小韦荣球菌和埃氏巨形球菌。3、通过检测发现体外瘤胃液对挥发性脂肪酸有一定的影响,对不同时间段的检测表明,ph先下降后上升;乙酸、丙酸呈下降趋势;乳酸浓度呈下降趋势;丁酸是先上升后下降。结论1、已明确高产丙酸菌对奶牛瘤胃丙酸生成量及能量代谢的有一定影响,揭示微生态制剂改善奶牛能量负平衡的作用机制2、从健康奶牛瘤胃中分离出高产丙酸菌株;通过酵母菌组合与高产丙酸菌株体外复合培养,筛选出高产丙酸菌株与酵母最优组合,即热带假丝酵母、酿酒酵母和产朊假丝酵母,以及三株丙酸生成菌,月形单胞菌菌液、小韦荣球菌和埃氏巨形球菌。研制出可改善奶牛能量负平衡的复合微生态制剂。
[Abstract]:Objective according to the characteristic that ruminants mainly rely on rumen fermentation, a highly effective and safe compound microecological preparation of rumen with propionic acid bacteria was developed, which can increase rumen propionic acid production and improve negative balance of energy in dairy cows. So as to solve the technical problems currently faced in production. Methods through isolation and identification of propionic acid-producing strains in rumen of healthy dairy cows, the superior combination of propionic acid-producing strains and yeast was screened, and the high efficiency was obtained. A non-polluting rumen microecological preparation with propionic acid-producing bacteria as the core technology in perinatal dairy cows. Results 1. Propionic acid producing bacteria were successfully isolated from rumen juice by isolation of anaerobic bacteria. The propionic acid producing bacteria were successfully identified by biochemical characteristics and 16s RNA cloning method. The best combination of propionic acid producing strain and feed yeast, namely Candida tropicalis, Saccharomyces cerevisiae and Candida utilis, and three strains of propionic acid producing bacteria, and three strains of propionic acid producing bacteria, and the liquid of three strains of propionic acid producing bacteria, and three strains of propionic acid producing bacteria, were screened. It was found that rumen fluid in vitro had a certain effect on volatile fatty acids (VFA). The results showed that ph decreased first and then increased at different time intervals. Acetic acid and propionic acid showed a decreasing trend; lactic acid concentration showed a downward trend; butyric acid increased first and then decreased. Conclusion 1. The effects of propionic acid-producing bacteria on rumen propionic acid production and energy metabolism in dairy cows have been clarified, and the mechanism of improving negative balance of energy by microecological agents has been revealed. 2. High-yield propionic acid strains have been isolated from the rumen of healthy cows. The optimal combination of Candida tropicalis, Saccharomyces cerevisiae and Candida utilis, three propionic acid producing strains, and three strains of propionic acid producing bacteria, the liquid of S. monocytogenes, were selected by combination of yeast and propionic acid-producing bacteria in vitro. Vecrococcus and Giant Ehrlich. A composite microecological agent was developed to improve the negative balance of energy in dairy cows.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823.5

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