香猪睾丸miR-449b和miR-222分子的发育性变化

发布时间：2019-03-03 15:45

【摘要】：为了阐明miR-449b和miR-222对贵州从江香猪睾丸发育的调节作用。运用qPCR方法检测香猪睾丸组织中miR-449b和miR-222表达量的发育性变化,通过miRSystem数据库、Kobas数据库对预测的靶基因进行信号转导通路富集分析。经克隆测序,得到香猪miR-222序列,与猪的相似性为100%,而且物种之间miR-449b和miR-222两个分子的保守性都很高。香猪睾丸中miR-449b于1~4月龄期间的表达量逐渐上升,4月龄时表达量达到峰值,之后下降;miR-222的表达量呈现相反的变化趋势,于1~4月龄期间表达量逐渐下降,1月龄时最高,4月龄时最低,之后的表达量有所回升;已知物种的miR-449b和miR-222序列高度保守;经预测miR-449b靶基因有420个,富集于卵母细胞减数分裂、Notch信号通路、Ras信号通路等100个途径(P0.05),miR-222的靶基因有429个,富集于细胞周期、Fox O信号通路、TGF-β信号通路等69个通路(P0.05);miR-449b和miR-222的靶基因共享的通路为Wnt信号通路、MAPK信号通路、雌激素信号通路等42个(P0.05)。
[Abstract]:In order to elucidate the regulatory effects of miR-449b and miR-222 on testicular development of Congjiang Xiang pigs in Guizhou Province. The developmental changes of miR-449b and miR-222 expression in testis tissue of Xiang pig were detected by qPCR. The predicted target genes were enriched by signal transduction pathway by miRSystem database and Kobas database. After cloning and sequencing, the sequence of Xiang pig miR-222 was 100% similar to that of pig, and the conservatism of miR-449b and miR-222 among species was very high. The expression of miR-449b in the testis of Xiang pig increased gradually from 1 to 4 months old, reached the peak at 4 months old, and then decreased. The expression of miR-222 decreased gradually at the age of 1-4 months, the highest at 1 month, the lowest at 4 months, and then increased, and the miR-449b and miR-222 sequences of the known species were highly conserved. It was predicted that there were 420target genes of miR-449b, which were enriched in meiosis of oocytes, Notch signaling pathway, Ras signaling pathway and so on (P0.05). 429 target genes of miR-222 were enriched in, Fox O signaling pathway of cell cycle. 69 pathways such as TGF- 尾 signaling pathway (P0.05); The target genes of miR-449b and miR-222 shared 42 pathways such as Wnt signal pathway, MAPK signal pathway, estrogen signal pathway and so on (P0.05).
【作者单位】：贵州大学动物科学学院;贵州大学省农业生物工程研究院;
【基金】：国家高技术研究发展计划(863计划)课题(2013AA1-02503) 贵州省农业攻关项目(黔科合NY[2013]3073号) 贵州省“百”层次创新型人才项目(黔科合人才2016-4012号) 国家自然科学基金(31672390)
【分类号】：S828

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