氮沉降增加对贝加尔针茅草原土壤微生物群落结构的影响
发布时间：2019-03-04 08:15
【摘要】：土壤微生物是草原土壤生态系统的重要组成部分。为研究氮沉降增加对草原土壤微生物群落结构的影响,以内蒙古贝加尔针茅草原为研究对象,开展连续6年(2010—2015年)模拟氮沉降试验,以N计算,设置:N0(0 kg·hm~(-2))、N50(50kg·hm~(-2))、N100(100 kg·hm~(-2))、N150(150 kg·hm~(-2))和N300(300 kg·hm~(-2))5个处理,采用磷脂脂肪酸(PLFA)技术测定0~10 cm土壤特征微生物PLFA生物标记数量并探讨土壤微生物群落结构对氮沉降的响应。结果表明:随氮添加量增大,土壤微生物总PLFAs、细菌PLFAs、革兰氏阳性细菌PLFAs、革兰氏阴性细菌PLFAs和放线菌PLFAs含量呈先升高后降低的趋势,均以N100(100 kg·hm~(-2))处理最高。土壤微生物群落PLFA标记的主成分分析显示,不同氮添加下土壤微生物PLFA标记有显著差异。相关分析表明,土壤革兰氏阳性菌、放线菌PLFA含量、G~+/G~-与土壤p H值呈显著负相关,土壤微生物总PLFAs、土壤细菌PLFAs、革兰氏阳性菌PLFAs、革兰氏阴性菌PLFAs、放线菌PLFAs和饱和脂肪酸PLFAs含量均与土壤速效磷含量呈显著正相关。综合研究表明,连续6年氮添加改变了贝加尔针茅草原土壤微生物群落结构,土壤p H值和土壤速效磷含量是驱动这种变化的主要因素。
[Abstract]:Soil microorganism is an important part of grassland soil ecosystem. In order to study the effect of nitrogen deposition on soil microbial community structure in steppe, the experiment of simulated nitrogen deposition in Stipa Baikal grassland in Inner Mongolia for 6 consecutive years (2010-2015) was carried out, calculated by N, and the effect of nitrogen deposition on soil microbial community structure was studied. Set: N0 (0 kg 路hm ~ (- 2), N _ 50 (50kg 路hm ~ (- 2), N _ 100) and N _ 300 (300 kg 路hm ~ (- 2) five treatments, N0 (0 kg 路hm ~ (- 2), N150 (150 kg 路hm ~ (- 2) and N300 (300 kg 路hm ~ (- 2). Phospholipid fatty acid (PLFA) technique was used to determine the number of microbial PLFA biomarkers in 0 ~ 10 cm soil and to investigate the response of soil microbial community structure to nitrogen deposition. The results showed that the contents of total PLFAs, bacteria PLFAs, Gram-positive bacteria PLFAs, Gram-negative bacteria PLFAs and actinomycetes PLFAs increased at first and then decreased with the increase of nitrogen content. The treatment of N 100 (100 kg 路hm ~ (- 2) was the highest. Principal component analysis (PCA) of PLFA markers in soil microbial communities showed that there were significant differences in soil microbial PLFA markers under different nitrogen additions. The correlation analysis showed that the contents of Gram positive bacteria and actinomycetes PLFA in soil were negatively correlated with the pH value of soil, and the total PLFAs, bacteria in soil were PLFAs, Gram positive bacteria PLFAs, Gram negative bacteria PLFAs,. The content of actinomycetes PLFAs and saturated fatty acid PLFAs were positively correlated with the content of available phosphorus in soil. The results showed that the soil microbial community structure was changed by nitrogen addition for 6 years. The pH value of soil and the content of available phosphorus in soil were the main factors driving this change.
【作者单位】： 农业部环境保护科研监测所;
【基金】：国家自然科学基金项目(31170435)
【分类号】：S812.2
本文编号：2434095
[Abstract]:Soil microorganism is an important part of grassland soil ecosystem. In order to study the effect of nitrogen deposition on soil microbial community structure in steppe, the experiment of simulated nitrogen deposition in Stipa Baikal grassland in Inner Mongolia for 6 consecutive years (2010-2015) was carried out, calculated by N, and the effect of nitrogen deposition on soil microbial community structure was studied. Set: N0 (0 kg 路hm ~ (- 2), N _ 50 (50kg 路hm ~ (- 2), N _ 100) and N _ 300 (300 kg 路hm ~ (- 2) five treatments, N0 (0 kg 路hm ~ (- 2), N150 (150 kg 路hm ~ (- 2) and N300 (300 kg 路hm ~ (- 2). Phospholipid fatty acid (PLFA) technique was used to determine the number of microbial PLFA biomarkers in 0 ~ 10 cm soil and to investigate the response of soil microbial community structure to nitrogen deposition. The results showed that the contents of total PLFAs, bacteria PLFAs, Gram-positive bacteria PLFAs, Gram-negative bacteria PLFAs and actinomycetes PLFAs increased at first and then decreased with the increase of nitrogen content. The treatment of N 100 (100 kg 路hm ~ (- 2) was the highest. Principal component analysis (PCA) of PLFA markers in soil microbial communities showed that there were significant differences in soil microbial PLFA markers under different nitrogen additions. The correlation analysis showed that the contents of Gram positive bacteria and actinomycetes PLFA in soil were negatively correlated with the pH value of soil, and the total PLFAs, bacteria in soil were PLFAs, Gram positive bacteria PLFAs, Gram negative bacteria PLFAs,. The content of actinomycetes PLFAs and saturated fatty acid PLFAs were positively correlated with the content of available phosphorus in soil. The results showed that the soil microbial community structure was changed by nitrogen addition for 6 years. The pH value of soil and the content of available phosphorus in soil were the main factors driving this change.
【作者单位】： 农业部环境保护科研监测所;
【基金】：国家自然科学基金项目(31170435)
【分类号】：S812.2
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