组胺受体拮抗剂对低致病性禽流感患鸡血液指标的影响

发布时间：2019-03-04 16:59

【摘要】：为了研究组胺受体拮抗剂对低致病性禽流感病毒(LPAIV)感染黑脚麻鸡后的患鸡血液学的影响,探讨组胺受体1(H1)、组胺受体2(H2)在低致病性禽流感发生发展过程中的作用,试验选用120只1日龄黑脚麻鸡,育雏至1月龄后每日早晚分别注射0.4 mL/kg的组胺受体拮抗剂或生理盐水,并通过滴鼻感染禽流感病毒H9N2(107EID50),具体分为4组,阴性组(灭菌生理盐水)、阳性组(灭菌生理盐水+H9N2)、扑尔敏(扑尔敏+H9N2)组和西咪替丁(西咪替丁+H9N2)组,分别在第37、44、51日龄采血,测定血液指标。结果显示:扑尔敏组血液中白细胞数量、淋巴细胞数量在37和51日龄时均极显著(P0.01)高于西咪替丁组和阳性组;第44日龄阳性组白细胞数量、淋巴细胞数量极显著(P0.01)高于阴性组和扑尔敏组。37和51日龄时阳性组红细胞数量、血红蛋白浓度、红细胞体积和血小板含量均极显著(P0.01)低于阴性组和扑尔敏组;44日龄时阳性组红细胞数量、红细胞体积和血红蛋白浓度极显著(P0.01)低于阴性组和扑尔敏组,而阳性组和西咪替丁组血小板含量极显著(P0.01)高于阴性组和扑尔敏组。结果表明:扑尔敏能够稳定低致病性禽流感患鸡血液中白细胞数量、淋巴细胞数量、红细胞相关指标和血小板数量,西咪替丁效果不显著。
[Abstract]:In order to study the effects of histamine receptor antagonists on the hematology of chickens infected with low pathogenic avian influenza virus (LPAIV), histamine receptor 1 (H1) was studied. The effect of histamine receptor 2 (H2) on the occurrence and development of low pathogenic avian influenza. 120 1-day-old black-footed hemp chickens were injected with 0.4 mL/kg histamine receptor antagonist or saline each day and night after 1 month of age. Influenza virus H9N2 (107EID50) was infected intranasally and divided into 4 groups: negative group (sterilized saline), positive group (sterilized saline H9N2), chlorphenamine (H9N2) group and cimetidine (cimetidine H9N2) group. Blood samples were collected at the age of 37, 44 and 51 days, and blood indexes were measured. The results showed that the number of white blood cells and lymphocytes in the paracetamol group were significantly higher than those in the cimetidine group and the positive group at 37 and 51 days old (P0.01). The number of leukocytes and lymphocytes in the 44-day-old positive group were significantly higher than those in the negative group and paracetamol group (P0.01). Red blood cell volume and platelet content were significantly lower (P0.01) than those in negative group and paracetamol group. At the age of 44 days, the number of erythrocytes, the volume of red blood cells and the concentration of hemoglobin in the positive group were significantly lower than those in the negative group and paracetamol group (P0.01). The platelet contents in positive group and cimetidine group were significantly higher than those in negative group and paracetamol group (P0.01). The results showed that chlorphenamine could stabilize the white blood cell number, lymphocyte number, red blood cell related index and platelet number, and cimetidine had no significant effect on the low pathogenic avian influenza chicken.
【作者单位】：贵州大学动物科学学院;
【基金】：基金项目:黔科合NY字(2016)2514号
【分类号】：S858.31

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