艾蒿水提物对肉仔鸡血清细胞因子含量及小肠诱导型一氧化氮合成酶mRNA表达量的影响

发布时间：2019-03-07 12:15

【摘要】：本试验旨在研究饲粮中添加不同水平的艾蒿水提物对肉仔鸡血清细胞因子含量及小肠诱导型一氧化氮合成酶(i NOS)mRNA表达量的影响。选取192只健康的1日龄爱拔益加(AA)肉仔鸡,随机分为4个组,每组6个重复,每个重复8只鸡。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加500、1 000、2 000 mg/kg艾蒿水提物的试验饲粮。试验期42 d。结果表明:1)与对照组相比,21日龄时,2 000 mg/kg添加组的血清白细胞介素-1β(IL-1β)、白细胞介素-2(IL-2)和白细胞介素-4(IL-4)的含量显著升高(P0.05),500 mg/kg添加组的血清IL-4含量和1 000 mg/kg添加组的血清IL-2含量极显著升高(P0.01);42日龄时,500 mg/kg添加组的血清IL-2含量显著升高(P0.05)。2)与对照组相比,21日龄时,500 mg/kg添加组的血清一氧化氮(NO)含量和i NOS活性及十二指肠、空肠和回肠的i NOS mRNA表达量差异不显著(P0.05),1 000 mg/kg添加组的血清NO含量和回肠i NOS mRNA表达量显著升高(P0.05),2 000 mg/kg添加组的回肠i NOS mRNA表达量极显著升高(P0.01);42日龄时,500、1 000和2 000 mg/kg添加组血清NO含量和i NOS活性及空肠i NOS mRNA表达量显著或极显著升高(P0.05或P0.01)。由此可见,饲粮中添加艾蒿水提物可以促进肉仔鸡免疫细胞因子的分泌和NO的生成,且与i NOS mRNA的表达增强有关。
[Abstract]:The purpose of this study was to study the effects of different levels of water extract of Artemisia artemisia on serum cytokine content and the expression of inducible nitric oxide synthase (i NOS) mRNA) in small intestine of broilers. One hundred and twenty-two healthy 1-day-old Alba Yi plus (AA) broilers were randomly divided into 4 groups with 6 replicas in each group and 8 chickens in each group. The control group was fed with basic diet, the experimental group was fed with 500, 1 000, 2 000 mg/kg water extract of artemisia in the basal diet, and the experimental diet was fed with water extract of Artemisia artemisia. The experimental period is 42 d. The results showed that: 1) compared with the control group, at 21 days old, the serum interleukin-1 尾 (IL-1 尾) in the 2 000 mg/kg-treated group was significantly higher than that in the control group. The levels of interleukin-2 (IL-2) and interleukin-4 (IL-4) were significantly increased (P0.05). The levels of serum IL-4 and IL-2 in 500 mg/kg group and 1 000 mg/kg addition group were significantly higher than those in control group (P0.01). At the age of 42 days, the content of serum IL-2 in the group supplemented with 500 mg/kg was significantly higher than that in the control group (P0.05). Compared with the control group, at the age of 21 days, the content of serum nitric oxide (NO) and I NOS activity in the group supplemented with 500 mg/kg and the activity of duodenum were significantly increased. There was no significant difference in the expression of i NOS mRNA between jejunum and ileum (P0.05). The content of serum NO and the expression of i NOS mRNA in ileum were significantly increased in the 1 000 mg/kg group (P0.05). The expression of i NOS mRNA in ileum of 2 000 mg/kg-treated group was significantly higher than that of control group (P 0.01). At the age of 42 days, the serum NO content, I NOS activity and i NOS mRNA expression in jejunum were significantly increased in 500, 1 000 and 2 000 mg/kg groups (P0.05 or P0.01). It can be seen that the addition of Artemisia artemisia water extract can promote the secretion of immune cytokines and the production of NO in broilers, which is related to the increase of i NOS mRNA expression.
【作者单位】：内蒙古农业大学动物科学学院;
【基金】：国家自然科学基金资助项目(31660674)
【分类号】：S831.5

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