一株产纤维素酶的解淀粉芽孢杆菌的分离鉴定及其酶促反应适宜条件初步研究
发布时间：2019-03-07 11:00
【摘要】：为了分离、筛选产纤维素酶益生菌,确定其酶促反应适宜条件,利用羧甲基纤维素钠等作为筛选培养基,结合刚果红染色法,从玉米青贮饲料样品中筛选产纤维素酶益生菌,并通过形态学和系统进化树方法鉴定分离菌。同时对培养时间、pH和温度等酶促反应条件进行了研究。结果表明,筛选出1株可降解羧甲基纤维素,并产生清晰透明圈的菌株。经形态学观察和16SrRNA基因序列分析,鉴定该菌为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。粗酶液中滤纸酶活为1.5U/mL,外切葡聚糖酶活为1.3U/mL,内切葡聚糖酶活为6.4U/mL,β-葡萄糖苷酶活为2.3U/mL。酶促反应的适宜条件为pH 5.5,温度50℃,粗酶液为培养3d后发酵液上清。解淀粉芽孢杆菌分离株具有一定的产纤维素酶能力,在玉米秸秆生物降解与利用方面具有潜在的开发价值。
[Abstract]:In order to isolate and screen cellulase-producing probiotics and determine the suitable conditions for enzymatic reaction, cellulase-producing probiotics were screened from corn silage samples by using carboxymethyl cellulose sodium as screening medium and Congo red staining method. The isolated bacteria were identified by morphological and phylogenetic tree methods. At the same time, the conditions of enzymatic reaction, such as culture time, pH and temperature, were studied. The results showed that a strain which could degrade carboxymethyl cellulose and produce clear transparent circle was screened out. The strain was identified as Bacillus amylase (Bacillus amyloliquefaciens). By morphological observation and sequence analysis of 16SrRNA gene. The activity of filter paper, exoglucanase, endoglucanase and 尾-glucosidase in crude enzyme solution was 1.5U / mL, 1.3U / mL, 6.4U / mL and 2.3U / mL, respectively, and that of 尾-glucosidase was 1.3U / mL, 1.3U / mL, 6.4U / mL and 2.3U / mL respectively. The optimum conditions for enzymatic reaction were pH 5.5, temperature 50 鈩，
本文编号：2436056
[Abstract]:In order to isolate and screen cellulase-producing probiotics and determine the suitable conditions for enzymatic reaction, cellulase-producing probiotics were screened from corn silage samples by using carboxymethyl cellulose sodium as screening medium and Congo red staining method. The isolated bacteria were identified by morphological and phylogenetic tree methods. At the same time, the conditions of enzymatic reaction, such as culture time, pH and temperature, were studied. The results showed that a strain which could degrade carboxymethyl cellulose and produce clear transparent circle was screened out. The strain was identified as Bacillus amylase (Bacillus amyloliquefaciens). By morphological observation and sequence analysis of 16SrRNA gene. The activity of filter paper, exoglucanase, endoglucanase and 尾-glucosidase in crude enzyme solution was 1.5U / mL, 1.3U / mL, 6.4U / mL and 2.3U / mL, respectively, and that of 尾-glucosidase was 1.3U / mL, 1.3U / mL, 6.4U / mL and 2.3U / mL respectively. The optimum conditions for enzymatic reaction were pH 5.5, temperature 50 鈩，
本文编号：2436056
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