上海市肉鸽沙门氏菌的分离鉴定及流行血清型和耐药性分析

发布时间：2019-03-07 11:00

【摘要】：为了解上海市农贸市场肉鸽群体中沙门氏菌流行血清型和耐药情况,本试验于2011—2014年间从上海市各区农贸市场采集肉鸽新鲜粪便样本92份,用XLD平板和沙门氏菌属显色培养基分离疑似沙门氏菌,革兰氏染色镜检并进行生化试验,共获得沙门氏菌24株,分离率为26.1%。采用Kauffmann-White法和K-B纸片法对24株沙门氏菌进行血清型鉴定和16种抗生素的药敏试验。血清型鉴定显示,24株沙门氏菌可分为鼠伤寒(66.7%)、阿贡纳(25.0%)和科瓦利斯(8.3%)3种血清型。药敏试验结果显示,有75.0%(18株)的分离株表现出不同程度耐药,其中分离株对四环素和磺胺异唑耐药率最高,均达到62.5%,其次为链霉素(58.3%)、萘啶酸(50.0%)、氨苄西林(20.8%)。多重耐药菌株15株(62.5%),耐4种抗菌药物的菌株最多,占16.7%(7株)。另外,分离菌株对头孢吡肟、头孢噻肟、头孢噻甲羧肟、亚胺培南、氧氟沙星等5种药物的敏感率为100.0%,对奥格门丁和环丙沙星有12.5%的中介敏感率。本研究结果表明,上海市肉鸽中沙门氏菌的携带率较高,且菌株多重耐药现象较严重,这为食品中沙门氏菌的防控工作带来较大的挑战和风险,肉鸽沙门氏菌的流行和耐药情况应值得密切关注。
[Abstract]:In order to understand the prevalence of salmonella serotype and drug resistance in meat pigeons in Shanghai Agricultural Market, 92 samples of fresh feces of meat pigeons were collected from farmers' markets in Shanghai in 2011and 2014. XLD plate and Salmonella coloration medium were used to isolate suspected salmonella. Twenty-four strains of Salmonella were isolated by Gram staining microscope and biochemical test. The isolation rate was 26.1%. The serotypes of 24 strains of Salmonella and the drug susceptibility test of 16 antibiotics were determined by Kauffmann- White method and K-B paper method. Serotyping showed that 24 Salmonella strains could be divided into three serotypes: typhus typhimurium (66.7%), Agona (25.0%) and Kovalis (8.3%). The results of drug sensitivity test showed that 75.0% (18 strains) were resistant to tetracycline and sulfaisozole, and streptomycin (58.3%) was the second, with the highest rate of resistance to tetracycline and sulfazole, and the resistance rate to tetracycline and sulfaisozole was 62.5%, followed by streptomycin (58.3%). Naphthidine acid (50.0%) and ampicillin (20.8%). There were 15 (62.5%) multi-drug resistant strains, and 16.7% (7 strains) were resistant to 4 kinds of antibiotics. In addition, the susceptibility rate of the isolates to cefepime, cefotaxime, imipenem and ofloxacin was 100.0% and 12.5% to Ogmendine and ciprofloxacin, respectively. The susceptibility rate of the isolated strains to cefepime, cefotaxime, imipenem and ofloxacin was 100.0% and 12.5% respectively. The results showed that the carrier rate of Salmonella in meat pigeons in Shanghai was higher, and the multi-drug resistance of the strains was serious, which brought great challenge and risk to the prevention and control of Salmonella in food. The prevalence and drug resistance of Salmonella spp should be paid close attention.
【作者单位】：华南农业大学兽医学院广东省动物源性人兽共患病预防与控制重点实验室;上海市疾病预防控制中心;广州市白云区江高镇畜牧兽医站;
【基金】：公益性行业(农业)科研专项(201403054) 广东省大学生科技创新培育专项资金(pdjh2016a0072)
【分类号】：S858.39

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