以豆粕为基质的植物乳杆菌固态发酵菌剂的制备

发布时间：2019-03-24 11:25

【摘要】：研究了以豆粕(添加2%葡萄糖)为基质的植物乳杆菌固态发酵菌剂制备的最佳条件。以干燥后活菌数为指标,通过单因素和Box-Behnken响应面实验设计对发酵工艺进行优化,对豆粕发酵前后的主要营养成分及抗营养因子含量进行测定,并最终确定了烘干条件。结果表明,在发酵温度30.6℃、接种量4.45%、加水比1∶0.61、发酵时间46.40 h时,活菌数为最高达到9.94 lg(CFU/g);粗蛋白、酸溶蛋白、游离氨基酸总量和蛋白质消化率较发酵前分别提高了4.55%、1.24%、50.47 mg/g和4.21%;胰蛋白酶抑制因子、脲酶和植酸较发酵前分别降低了94.59%、91.64%和30.22%;最佳干燥条件为热风干燥50℃、4 h,菌剂活菌总数为9.88 lg(CFU/g)。
[Abstract]:The optimum conditions for the preparation of solid-state fermentation agent of Lactobacillus plantarum based on soybean meal (2% glucose) were studied. Taking the number of living bacteria after drying as an index, the fermentation process was optimized by single factor and Box-Behnken response surface experiment design. The main nutrient composition and anti-nutritive factor content of soybean meal before and after fermentation were determined, and the drying conditions were finally determined. The results showed that when the fermentation temperature was 30.6 鈩，

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