植物乳杆菌对玉米秸秆和水稻秸秆体外发酵特性的影响

发布时间：2019-03-27 21:02

【摘要】：本试验旨在探讨植物乳杆菌(Lactobacillus plantarum)对玉米秸秆和水稻秸秆奶牛瘤胃体外发酵特性的影响。采用单因子随机区组试验设计,分别以玉米秸秆和水稻秸秆为发酵底物,分析不同添加水平[0(对照)、0.25×107、0.50×107和0.75×107CFU/m L]植物乳杆菌对发酵底物体外发酵产气量(1、2、4、6、12、24、36、48 h)、产气参数、干物质降解率(DMD)、中性洗涤纤维降解率(NDFD)、发酵液挥发性脂肪酸(VFA)、氨态氮(NH3-N)浓度及p H的影响。结果表明:添加植物乳杆菌能显著提高玉米秸秆发酵初期产气速率和产气量(1~24 h)(P0.05),以添加0.75×107CFU/m L效果最为理想;添加植物乳杆菌能显著提高水稻秸秆体外发酵后期(36~48 h)产气量(P0.05),而以添加0.25×107CFU/m L效果最为理想。随着植物乳杆菌添加水平的增加,2种底物体外发酵液NH3-N浓度均呈现显著的线性增加效应(P0.05)。不同植物乳杆菌添加水平对2种底物体外发酵NDFD、DMD、发酵液VFA(乙酸、丙酸、异丁酸、丁酸和戊酸)浓度以及p H均无显著影响(P0.05)。由试验结果推断,添加植物乳杆菌能促进玉米秸秆和水稻秸秆体外发酵及其氮代谢,同时对维持p H的稳定平衡具有积极作用,最佳添加水平分别为0.75×107和0.25×107CFU/m L。
[Abstract]:The aim of this experiment was to investigate the effects of Lactobacillus botanica (Lactobacillus plantarum) on rumen fermentation characteristics of corn and rice stalks in dairy cows in vitro. Using single factor random block design, corn straw and rice straw were used as fermentation substrates to analyze different adding levels [0 (control)]. 0.25 脳 10 ~ 7, 0.50 脳 10 ~ 7 and 0.75 脳 107CFU/m / L] the in vitro gas production of Lactobacillus botanica to the fermentation substrate (1, 2, 4, 6, 12, 24, 36, 48 h), gas production parameters, dry matter degradation rate (DMD), neutral detergent degradation rate (NDFD),) Effect of volatile fatty acid (VFA), ammonia nitrogen (NH3-N) concentration and pH in fermentation broth. The results showed that Lactobacillus botanica could significantly increase the gas production rate and gas production at the initial stage of corn straw fermentation (1 脳 107CFU/m / L, P 0.05), and the best effect was 0.75 脳 107CFU/m / L; The addition of Lactobacillus botanica could significantly increase the gas production of rice straw at the end of in vitro fermentation (36 h / 48 h) (P0.05), and the effect of adding 0.25 脳 107CFU/m / L was the best. With the increase of Lactobacillus botanica addition level, the concentration of NH3-N in fermentation broth of the two substrates in vitro showed a significant linear increase (P0.05). The concentration of VFA (acetic acid, propionic acid, isobutyric acid, butyric acid and valproic acid) and pH in NDFD,DMD, fermentation broth fermented by two substrates were not significantly affected by different levels of Lactobacillus botanica (P0.05). The results showed that Lactobacillus botanica could promote the in vitro fermentation and nitrogen metabolism of corn and rice stalks, and had a positive effect on maintaining the stable balance of pH. The optimum addition levels were 0.75 脳 10 ~ 7 and 0.25 脳 107CFU/m 路L ~ (- 1), respectively.
【作者单位】：湖南农业大学动物科学技术学院;中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室湖南省畜禽健康养殖工程技术中心农业部中南动物营养与饲料科学观测实验站;西藏自治区农牧科学院畜牧兽医研究所;湖南畜禽安全生产协同创新中心;
【基金】：娟姗牛生产性能与乳品质提升营养调控关键技术研究(西藏自治区财政专项) 国家“十二五”科技支撑计划(2012BAD14B17) 国家自然科学基金(31001024) 中国科学院亚热带农业生态研究所青年人才领域项目(ISACX-LYQY-QN-1105)
【分类号】：S816

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