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绝食对九龙牦牛体重、血清激素和生化指标的影响

发布时间:2019-04-02 04:57
【摘要】:本试验旨在研究冷季绝食应激时九龙牦牛体重、血清激素和生化指标的变化。选择8头健康无病、生长发育良好的2岁九龙牦牛8头,体重为(90.75±8.66)kg,按体重相近的原则随机分为对照组和绝食组,每组4个重复,每个重复1头牛。对照组正常饲喂,绝食组牦牛做断料处理。试验期7 d。分别在试验开始后的第1、3、5、7天称重和通过颈静脉采血。结果表明:绝食7 d后牦牛的体重显著降低(P0.05),血清中葡萄糖、胰岛素含量显著下降(P0.05),血清非酯化脂肪酸、甘油三酯、脂联素和胰高血糖素含量显著升高(P0.05),血清中糖异生前体物质丙酸、乳酸的含量显著降低(P0.05),而血清中来源于体储降解的甘油、β-羟丁酸的含量显著升高(P0.05),血清中糖异生关键酶丙酮酸羧化酶、果糖1,6二磷酸酶、磷酸烯醇式丙酮酸羧激酶、葡萄糖六磷酸酶的活性均显著升高(P0.05)。结果提示,绝食7 d后牦牛开始大量动用体储,提高糖异生代谢过程,维持机体代谢的正常需要,保证血清葡萄糖含量的稳定以维持生命。
[Abstract]:The purpose of this experiment was to study the changes of body weight, serum hormone and biochemical indexes of Kowloon yak during cold season hunger strike. Eight 2-year-old 2-year-old Kowloon yaks with a body weight of (90.75 卤8.66) kg, were randomly divided into control group and hunger strike group according to the principle of similar body weight. The control group was fed with normal diet, and the fasting group was treated with cut-off material. 7 d. Weighing and collecting blood through jugular vein 1, 3, 5, 7 days after the beginning of the trial. The results showed that after 7 days of hunger strike, the body weight of yak decreased significantly (P0.05), the content of glucose and insulin in serum decreased significantly (P0.05), the serum non-esterified fatty acids, triglycerides, The levels of adiponectin and glucagon were significantly increased (P0.05), and the contents of propionic acid and lactic acid in serum were significantly decreased (P0.05), while the contents of glycerol, which originated from body storage and degradation, were significantly decreased (P0.05). The content of 尾-hydroxybutyric acid was significantly increased (P0.05), and the activities of glycosylation key enzymes pyruvate carboxylase, fructose-1, 6-diphosphatase, phosphoenolpyruvate carboxykinase and glucose hexaphase in serum were significantly increased (P0.05). The results indicated that yaks began to use a large amount of body storage after 7 days of hunger strike to improve the metabolic process of glucose, maintain the normal needs of metabolism, and ensure the stability of serum glucose content in order to maintain life.
【作者单位】: 四川农业大学动物营养研究所牛低碳养殖与安全生产四川省高校重点实验室;
【基金】:国家自然科学基金项目(31470121)
【分类号】:S823.85

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