基于RNA-Seq转录组测序技术揭示南方黄牛肉质嫩度调控相关的分子机制

发布时间：2019-04-02 17:33

【摘要】：为揭示南方黄牛肉质调控的分子机制并挖掘与肌肉嫩度相关的功能基因,本研究以1周岁左右的云岭牛、文山牛和中国西门塔尔牛为试验对象,屠宰后测定背最长肌不同排酸时间(0、1、2、3、5、7 d)下剪切力变化,揭示不同黄牛品种肌肉嫩度的差异,利用RNA-Seq技术对背最长肌进行比较转录组学分析,并结合生物信息学筛选与肌肉嫩度调控相关的信号通路及差异表达基因,最后利用q PCR进行验证。结果表明:在宰后成熟过程中,黄牛肌肉嫩度表现为明显的下降趋势,且在同一个成熟时间点下,剪切力值表现为西门塔尔牛文山牛云岭牛,表明云岭牛嫩度较好,而西门塔尔牛嫩度相对较差;云岭牛与西门塔尔牛背最长肌2组文库经测序后,筛选出3 198个差异表达基因,其中云岭牛上调基因1 750个,KEGG功能注释发现2个与肉质调控相关的重要信号通路:脂肪细胞因子信号通路和内质网蛋白加工通路,结合q PCR验证、基因表达水平与剪切力值相关性分析,最终筛选出Leptin和CAPN12个可能肌肉嫩度相关的功能基因。本研究筛选与鉴定出南方黄牛肌肉嫩度相关信号通路及其关键基因,为今后南方黄牛肉质遗传改良奠定理论基础。
[Abstract]:In order to reveal the molecular mechanism of meat quality regulation in southern yellow cattle and excavate functional genes related to muscle tenderness, Yunling cattle, Wenshan cattle and Chinese Simmental cattle aged about one year were selected as experimental subjects. In order to reveal the difference of muscle tenderness of different yellow cattle breeds, the muscle tenderness of the longest dorsum muscle was analyzed by RNA-Seq technique, and the changes of shear stress at different times (0, 1, 2, 3, 5, 7 days) were measured after slaughter, and the muscle tenderness of the longissimus dorsi muscle was compared with that of the longissimus dorsi muscle by means of RT-PCR. Combined with bioinformatics, signal pathways and differentially expressed genes related to muscle tenderness regulation were screened and verified by Q-PCR. The results showed that the muscle tenderness of yellow cattle decreased obviously during postmortem ripening, and at the same mature time point, the shear force was that of Simmental Niuwenshan Niu Yunling cattle, which indicated that Yunling cattle had better tenderness. The tenderness of Simmental cattle is relatively poor; After sequencing, 3 198 differentially expressed genes were screened between Yunling cattle and Simmental cattle. Among them, 1,750 genes were up-regulated in Yunling cattle. KEGG functional annotation found two important signaling pathways related to meat quality regulation: adipocytokine signaling pathway and endoplasmic reticulum protein processing pathway. Combined with Q-PCR verification, the correlation between gene expression level and shear force was analyzed. Finally, the functional genes of Leptin and CAPN12 which may be related to muscle tenderness were screened out. In this study, the signal pathway and key genes related to muscle tenderness of southern yellow cattle were screened and identified, which laid a theoretical foundation for genetic improvement of meat quality of southern yellow cattle in the future.
【作者单位】：扬州大学旅游烹饪学院;云南省草地与动物研究院;
【基金】：863国家高技术研究发展计划项目(2013AA102505-5)
【分类号】：S823.2

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