低剂量多巴胺对肉鸡肾脏钠钾氯共转运体表达的影响
发布时间：2019-04-04 10:31
【摘要】：为研究低剂量多巴胺对肉鸡肾脏钠钾氯共转运体(NKCC2)表达的影响,按常规条件饲养AA肉鸡40羽,25日龄时随机分为对照组和试验组,对照组肉鸡经静脉注射5μg/kg/min 0.9%生理盐水,试验组肉鸡按相同方法注射等体积低剂量左旋多巴胺,并采用组织形态学观察、血尿生化检测、蛋白质免疫印迹试验和RT-q PCR等方法检测肾组织形态、各生化指标的变化、NKCC2蛋白水平和mRNA水平的变化。结果显示:与对照组相比,试验组在给予低剂量左旋多巴胺后,尿素氮(BUN)、肌酐(Cr)及平均动脉压(m PAP)水平均未发生明显改变(P0.05);低剂量左旋多巴胺持续给药后,肉鸡肾小管上皮细胞胞膜NKCC2蛋白水平显著降低(P0.05),细胞质NKCC2蛋白显著升高(P0.05);然而NKCC2 mRNA水平未发生明显变化(P0.05)。结论:在不影响肾小球滤过率及血压前提下,低剂量多巴胺能够引起肉鸡机体持续的利钠利尿作用,其机制与NKCC2蛋白的穿梭作用有关。
[Abstract]:In order to study the effect of low dose dopamine on the expression of sodium, potassium and chloride cotransporter (NKCC2) in kidney of broilers, 40 AA broilers were fed with conventional conditions and randomly divided into control group and experimental group at the age of 25 days. In the control group, 5 渭 g / kg/min 0.9% saline was injected intravenously, and the same volume low dose of L-dopamine was injected into the broilers of the experimental group by the same method. The histological observation and biochemical examination of hematuria were performed in the experimental group. Western blot and RT-q PCR were used to detect the changes of renal tissue morphology, biochemical indexes, NKCC2 protein level and mRNA level. The results showed that compared with the control group, the levels of urea nitrogen (BUN), creatinine (Cr) and mean arterial pressure (m PAP) did not change significantly in the experimental group after low dose of L-dopamine (P0.05). After continuous administration of low dose of L-dopamine, the level of membrane NKCC2 protein in renal tubular epithelial cells of broilers was significantly decreased (P0.05), and the level of cytoplasmic NKCC2 protein was significantly increased (P0.05), but the level of NKCC2 mRNA did not change significantly (P0.05). Conclusion: low dose dopamine can induce continuous natriuretic diuresis in broilers without affecting glomerular filtration rate and blood pressure, and the mechanism is related to the shuttle effect of NKCC2 protein.
【作者单位】： 华中农业大学动物医学院;湖北省农业科学院畜牧兽医研究所;
【基金】：国家自然科学基金青年科学基金(31502132) 湖北省自然科学基金计划项目(2014CFB244) 中央高校基本科研业务费专项基金(2011PYO78)
【分类号】：S831
[Abstract]:In order to study the effect of low dose dopamine on the expression of sodium, potassium and chloride cotransporter (NKCC2) in kidney of broilers, 40 AA broilers were fed with conventional conditions and randomly divided into control group and experimental group at the age of 25 days. In the control group, 5 渭 g / kg/min 0.9% saline was injected intravenously, and the same volume low dose of L-dopamine was injected into the broilers of the experimental group by the same method. The histological observation and biochemical examination of hematuria were performed in the experimental group. Western blot and RT-q PCR were used to detect the changes of renal tissue morphology, biochemical indexes, NKCC2 protein level and mRNA level. The results showed that compared with the control group, the levels of urea nitrogen (BUN), creatinine (Cr) and mean arterial pressure (m PAP) did not change significantly in the experimental group after low dose of L-dopamine (P0.05). After continuous administration of low dose of L-dopamine, the level of membrane NKCC2 protein in renal tubular epithelial cells of broilers was significantly decreased (P0.05), and the level of cytoplasmic NKCC2 protein was significantly increased (P0.05), but the level of NKCC2 mRNA did not change significantly (P0.05). Conclusion: low dose dopamine can induce continuous natriuretic diuresis in broilers without affecting glomerular filtration rate and blood pressure, and the mechanism is related to the shuttle effect of NKCC2 protein.
【作者单位】： 华中农业大学动物医学院;湖北省农业科学院畜牧兽医研究所;
【基金】：国家自然科学基金青年科学基金(31502132) 湖北省自然科学基金计划项目(2014CFB244) 中央高校基本科研业务费专项基金(2011PYO78)
【分类号】：S831
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