鹿场环境中细菌的分布及耐药性调查

发布时间：2019-04-24 01:08

【摘要】：为探究辽宁省西丰县鹿场环境中细菌的分布及耐药性情况,本试验采集了鹿场粪便、饮水、空气、饲料等样品,进行了细菌的分离培养及鉴定,对分离到的菌株16SrDNA基因进行PCR扩增、测序分析,并对分选出的致病菌和条件致病菌进行药敏试验。结果显示,所测样品共分离纯化出27株菌,公鹿精饲料、母鹿精饲料、粗饲料和水的含菌量分别为1.0×10~5、8.7×10~4、2.7×10~7 CFU/g和2.9×10~4 CFU/mL,表明水和粗饲料不符合国家规定的养殖场环境卫生要求;16SrDNA基因序列在NCBI比对后,鉴定出有大肠埃希菌、志贺氏菌等15种不同菌株,其中致病菌和条件致病菌共13种,这13种细菌对呋喃唑酮、多黏菌素B和头孢氨苄等耐药,对头孢曲松、环丙沙星和丁胺卡那等药物敏感。本试验结果可为鹿场细菌性疾病的防控及用药提供参考依据。
[Abstract]:In order to investigate the distribution and drug resistance of bacteria in deer farms in Xifeng County, Liaoning Province, samples of faeces, drinking water, air, feed and so on were collected in this experiment, and the bacteria were isolated, cultured and identified. The 16SrDNA gene of the isolated strain was amplified by PCR, sequenced, and tested for drug sensitivity of the selected and conditional pathogenic bacteria. The results showed that 27 strains of bacteria were isolated and purified. The bacterial contents of male deer concentrate feed, female deer concentrate feed, crude feed and water were 1.0 脳 10 ~ 5, 8.7 脳 10 ~ 4,2.7 脳 10 ~ 7 CFU/g and 2.9 脳 10 ~ 4 CFU/mL, respectively, and the bacterial contents of crude feed and water were 1.0 脳 10 ~ 5, 8.7 脳 10 ~ 4, 2.7 脳 10 ~ 7 CFU/mL, and 2.9 脳 10 ~ 4 CFU/mL, respectively. The results showed that the water and roughage did not meet the environmental hygiene requirements of farming farms stipulated by the state. 15 different strains of Escherichia coli and Shigella were identified after NCBI sequence comparison. Among them, 13 strains were pathogenic bacteria and conditional pathogens, which were resistant to furazolidone, polymyxin B and Cefalexin. Sensitive to ceftriaxone, ciprofloxacin and amikana. The results can be used as a reference for the prevention and control of bacterial diseases in deer farms.
【作者单位】：沈阳农业大学畜牧兽医学院;
【基金】：辽宁省三区人才支持项目(2013301004-2) 辽宁省大学生创新创业支持项目(20111070、20111423) 沈阳农业大学大学生创新创业支持项目
【分类号】：S858.25

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