脑红蛋白在西门塔尔牛主要器官组织中分布的研究
发布时间：2019-04-24 20:48
【摘要】：为了探寻脑红蛋白(Neuroglobin,NGB)在成年西门塔尔牛不同组织细胞的分布情况,选择健康成年西门塔尔牛5只,利用免疫组织化学染色SP法观察NGB在成年西门塔尔牛不同组织细胞的分布特征,结果显示:NGB在已检测的多数组织细胞中均有阳性表达,不同个体之间的NGB表达无明显差异。中枢神经系统中,NGB免疫阳性反应主要定位于神经细胞。大脑灰质中NGB阳性细胞广泛分布于大脑灰质第I至第VI层,免疫阳性反应强度在大脑灰质第I~VI层之间没有明显的差异,大脑白质区域也可见散在的NGB阳性细胞。嗅球的僧帽细胞层NGB免疫阳性反应相对强,胞体大。海马不同区域的锥体细胞中均可见NGB阳性表达。小脑灰质各层细胞中广泛分布有NGB阳性细胞,其中浦肯野细胞层免疫阳性反应强于颗粒层和分子层细胞,小脑白质中未见明显的阳性反应。在脊髓各区段,NGB阳性细胞主要分布于灰质,白质中阳性反应相对较弱。内分泌系统中,肾上腺皮质和髓质细胞均呈阳性反应,皮质中球状带细胞阳性反应最强,网状带次之,束状带最弱,髓质中反应普遍较皮质弱。腺垂体内腺细胞可见NGB阳性表达,表达部位主要在细胞质,神经垂体内神经纤维亦呈阳性反应。松果体中可见有广泛分布的NGB阳性细胞。在雄性生殖系统中,睾丸各级精母细胞和部分间质细胞均显示有NGB阳性表达,附睾输出小管和附睾管上皮细胞内亦可见NGB阳性表达,输精管未见表达。雌性生殖系统中,卵巢原始卵泡细胞及各级生长卵泡细胞的颗粒层中均有NGB阳性反应,子宫内膜上皮细胞和子宫腺导管上皮细胞呈NGB强阳性表达。成年西门塔尔牛消化系统中,多室胃前胃黏膜上皮细胞中均可见较强的NGB阳性反应,各肠段肠腺细胞中均有较弱的NGB阳性反应,但在十二指肠段的小肠腺细胞较十二指肠腺细胞的阳性表达强。肝脏胆小管可见有NGB阳性反应。胰腺中胰岛细胞及腺泡细胞均可见NGB阳性表达,表达部位主要在细胞质,其中胰岛细胞阳性反应较强。颌下腺的纹状管上皮细胞有NGB阳性表达。腮腺腺泡细胞和导管也均有较弱的NGB阳性反应。西门塔尔牛其他组织细胞,心肌纤维中可见较弱的NGB阳性表达。脾脏的红髓中可见NGB阳性细胞,白髓中未见表达。肺脏细支气管上皮细胞及肺泡上皮细胞中均可见较弱的NGB阳性表达,表达部位主要在胞质。肾脏的远曲小管、近曲小管和集合小管的上皮细胞均有较弱的NGB阳性表达,肾小球内未见有明显的NGB阳性反应。膀胱中未见NGB阳性反应。NGB在成年西门塔尔牛多种器官组织细胞中的广泛分布,提示NGB不仅在氧的利用及功能活动中发挥作用,在其它生理活动中可能也担负着重要的功能。
[Abstract]:In order to investigate the distribution of brain erythrin (Neuroglobin,NGB) in different tissues and cells of adult Simmental cattle, five healthy adult Simmental cattle were selected. The distribution of NGB in different tissues and cells of adult Simmental cattle was observed by immunohistochemical staining SP method. The results showed that NGB was positive in most of the detected tissues and cells, and there was no significant difference in the expression of NGB among different individuals. In the central nervous system, NGB immunoreactivity was mainly localized in nerve cells. The NGB positive cells in the gray matter were widely distributed in the layers I to VI of the gray matter. There was no significant difference in the intensity of the immunoreactive reaction between the layers I ~ VI in the gray matter, and the scattered NGB positive cells could also be seen in the white matter area of the brain. The NGB immunoreactivity in the cell layer of olfactory bulb was relatively strong and the cell body was large. The positive expression of NGB was found in pyramidal cells in different regions of hippocampus. NGB positive cells were widely distributed in all layers of cerebellar gray matter. The immunopositive reaction in Purkinje cell layer was stronger than that in granular layer and molecular layer, and there was no obvious positive reaction in cerebellar white matter. In all segments of spinal cord, NGB positive cells were mainly distributed in gray matter, while the positive reaction in white matter was relatively weak. In the endocrine system, the adrenal cortex and medulla cells were positive, the globular zone cells in the cortex were the strongest, the reticular zone was the second, the fascicular zone was the weakest, and the reaction in the medulla was generally weaker than that in the cortex. The positive expression of NGB was observed in adenohypophysis cells, mainly located in cytoplasm and nerve fibers in neurohypophysis. A wide range of NGB positive cells were found in pineal gland. In male reproductive system, the positive expression of NGB was observed in spermatocytes and some Leydig cells of testis. The positive expression of NGB was also observed in epididymal output tubule and epididymal duct epithelial cells, and there was no expression in vas deferens. In female reproductive system, NGB positive reaction was found in ovarian primordial follicular cells and granular layers of all grade growing follicular cells, and strong positive expression of NGB was observed in endometrial epithelial cells and glandular ductal epithelial cells of uterus. In the digestive system of adult Simmental cattle, strong positive reaction of NGB was observed in the epithelial cells of multiloculed forestomach mucosa, and weak positive reaction of NGB was found in the intestinal gland cells of each intestinal segment. However, the positive expression of small intestinal gland cells in duodenum segment was stronger than that in duodenum gland cells. The positive reaction of NGB was found in the bile duct of the liver. The positive expression of NGB was found in pancreatic islet cells and acinar cells, mainly in cytoplasm, and the positive reaction of pancreatic islet cells was stronger than that of pancreatic acinar cells. There was positive expression of NGB in the striate duct epithelial cells of submandibular gland. Parotid acinar cells and ductus were also weakly positive for NGB. In other tissues and cells of Simmental cattle, weak positive expression of NGB was found in myocardial fibers. NGB positive cells were found in the red pulp of spleen, but no expression was found in the white pulp. Weak positive expression of NGB was found in bronchiolar epithelial cells and alveolar epithelial cells, mainly in cytoplasm. The epithelial cells of distal convoluted tubule, proximal convoluted tubule and collecting tubule all showed weak positive expression of NGB, but no obvious positive reaction of NGB was found in glomeruli. There was no NGB positive reaction in bladder. The extensive distribution of NGB in many organs, tissues and cells of adult Simmental cattle suggested that NGB not only played an important role in oxygen utilization and functional activities, but also played an important role in other physiological activities.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823
本文编号：2464773
[Abstract]:In order to investigate the distribution of brain erythrin (Neuroglobin,NGB) in different tissues and cells of adult Simmental cattle, five healthy adult Simmental cattle were selected. The distribution of NGB in different tissues and cells of adult Simmental cattle was observed by immunohistochemical staining SP method. The results showed that NGB was positive in most of the detected tissues and cells, and there was no significant difference in the expression of NGB among different individuals. In the central nervous system, NGB immunoreactivity was mainly localized in nerve cells. The NGB positive cells in the gray matter were widely distributed in the layers I to VI of the gray matter. There was no significant difference in the intensity of the immunoreactive reaction between the layers I ~ VI in the gray matter, and the scattered NGB positive cells could also be seen in the white matter area of the brain. The NGB immunoreactivity in the cell layer of olfactory bulb was relatively strong and the cell body was large. The positive expression of NGB was found in pyramidal cells in different regions of hippocampus. NGB positive cells were widely distributed in all layers of cerebellar gray matter. The immunopositive reaction in Purkinje cell layer was stronger than that in granular layer and molecular layer, and there was no obvious positive reaction in cerebellar white matter. In all segments of spinal cord, NGB positive cells were mainly distributed in gray matter, while the positive reaction in white matter was relatively weak. In the endocrine system, the adrenal cortex and medulla cells were positive, the globular zone cells in the cortex were the strongest, the reticular zone was the second, the fascicular zone was the weakest, and the reaction in the medulla was generally weaker than that in the cortex. The positive expression of NGB was observed in adenohypophysis cells, mainly located in cytoplasm and nerve fibers in neurohypophysis. A wide range of NGB positive cells were found in pineal gland. In male reproductive system, the positive expression of NGB was observed in spermatocytes and some Leydig cells of testis. The positive expression of NGB was also observed in epididymal output tubule and epididymal duct epithelial cells, and there was no expression in vas deferens. In female reproductive system, NGB positive reaction was found in ovarian primordial follicular cells and granular layers of all grade growing follicular cells, and strong positive expression of NGB was observed in endometrial epithelial cells and glandular ductal epithelial cells of uterus. In the digestive system of adult Simmental cattle, strong positive reaction of NGB was observed in the epithelial cells of multiloculed forestomach mucosa, and weak positive reaction of NGB was found in the intestinal gland cells of each intestinal segment. However, the positive expression of small intestinal gland cells in duodenum segment was stronger than that in duodenum gland cells. The positive reaction of NGB was found in the bile duct of the liver. The positive expression of NGB was found in pancreatic islet cells and acinar cells, mainly in cytoplasm, and the positive reaction of pancreatic islet cells was stronger than that of pancreatic acinar cells. There was positive expression of NGB in the striate duct epithelial cells of submandibular gland. Parotid acinar cells and ductus were also weakly positive for NGB. In other tissues and cells of Simmental cattle, weak positive expression of NGB was found in myocardial fibers. NGB positive cells were found in the red pulp of spleen, but no expression was found in the white pulp. Weak positive expression of NGB was found in bronchiolar epithelial cells and alveolar epithelial cells, mainly in cytoplasm. The epithelial cells of distal convoluted tubule, proximal convoluted tubule and collecting tubule all showed weak positive expression of NGB, but no obvious positive reaction of NGB was found in glomeruli. There was no NGB positive reaction in bladder. The extensive distribution of NGB in many organs, tissues and cells of adult Simmental cattle suggested that NGB not only played an important role in oxygen utilization and functional activities, but also played an important role in other physiological activities.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823
【参考文献】
相关期刊论文 前1条
1 尚爱加;周定标;孟祥辉;阎雅凤;;脑红蛋白在成年大鼠脊髓中的分布[J];解剖学报;2006年05期
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