FSH及Wnt信号通路对绵羊卵泡颗粒细胞的增殖及雌激素分泌的影响

发布时间：2019-04-26 16:32

【摘要】：旨在研究Wnt信号通路对绵羊卵泡颗粒细胞增殖及雌激素分泌的影响,探究其是否与促卵泡素(Follicular stimulating hormone,FSH)有关。在添加有FSH以及不同浓度Wnt信号通路抑制剂IWR-1的培养基中培养绵羊卵巢卵泡颗粒细胞168h,利用Guava ViaCount?Reagent测定细胞数目的变化,利用绵羊ELISA试剂盒检测雌激素浓度的变化,利用qRT-PCR检测FSH靶基因CYP19和CCND2的相对表达量差异。结果表明,无论有无FSH,抑制剂IWR-1使颗粒细胞数目显著减少(P0.05);当有FSH时,抑制剂IWR-1的添加导致雌激素浓度显著降低(P0.05)。IWR-1的浓度为1.0μmol·L-1时,对颗粒细胞的抑制效果最明显。IWR-1还导致CYP19和CCND2mRNA的表达量降低。综上表明,Wnt信号通路对绵羊卵泡颗粒细胞的生物学功能具有促进作用,并且这种促进作用可能与FSH有关。
[Abstract]:The aim of this study was to investigate the effect of Wnt signaling pathway on the proliferation and estrogen secretion of ovine follicular granulosa cells, and to explore whether it is related to follicle stimulating hormone (Follicular stimulating hormone,FSH). Ovine follicular granulosa cells were cultured in the medium supplemented with FSH and different concentration of Wnt signaling pathway inhibitor IWR-1 for 168 hours. The changes of cell number and estrogen concentration were measured by Guava ViaCount?Reagent and ELISA kit, respectively. The relative expression of FSH target gene CYP19 and CCND2 was detected by qRT-PCR. The results showed that the number of granulosa cells was significantly decreased with or without FSH, inhibitor IWR-1 (P0.05). At the concentration of 1.0 渭 mol 路L-1 of IWR-1, the inhibitory effect on granulosa cells was the most obvious. The expression of CYP19 and CCND2mRNA was also decreased by IWR-1 when the concentration of FSH was 1.0 渭 mol 路L-1. The concentration of estrogens decreased significantly with the addition of IWR-1 (P0.05). The results indicated that Wnt signaling pathway could promote the biological function of ovine follicular granulosa cells, and this effect might be related to FSH.
【作者单位】：山西农业大学动物科技学院;山西农业大学生命科学学院;
【基金】：国家自然科学基金(31172211) 山西省回国留学人员科研资助项目(2014-重点5) 山西农业大学科研管理费资助重大项目和标志性成果培育项目(71060003)
【分类号】：S826

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