锰中毒导致鸡免疫器官免疫和炎性损伤的研究

发布时间：2019-05-09 04:00

【摘要】：锰(Manganese,Mn)是自然界中广泛分布的一种元素,锰在生命活动的不同环节都表现出生物学功能,与酶的合成、三大物质代谢、造血、免疫和生殖功能有直接的关系。锰是机体必不可少营养元素,但过量锰对生物体有害。锰对神经、生殖、免疫系统造成的损伤已被证实。但锰对鸡免疫系统细胞因子和炎症因子影响的研究还很少见。本研究通过建立鸡锰中毒模型,探讨锰中毒对鸡免疫器官细胞因子和炎症因子的影响。7日龄180只海蓝蛋公鸡分为4组,对照组饲喂标准饲料,3个处理组饲喂标准饲料分别添加不同浓度的Mn Cl2(饲料中含锰的浓度分别为600、900和1800 mg/kg)。在锰处理30、60和90天后,分别采集法氏囊,胸腺和脾脏,测定三个免疫器官中IL-4、IL-7、IL-10、IL-17、TGF-β4、IFN-γ、NF-κB、TNF-α、COX-2、PGE2和iNOSm RNA表达。采用单因素方差分析方法,分析了不同组间检测因子的差异性,并运用多元相关分析、主成分分析、聚类分析三个方法分析检测的细胞因子之间的关系,试验得出如下结论:1.30天和60天锰中毒上调了法氏囊、胸腺和脾脏NO含量、iNOS活力、IL-4、IL-7、IL-10、IL-17、TGF-β4、NF-κB、TNF-α、COX-2、PGE2和iNOS mRNA表达,抑制了IFN-γm RNA表达,90天锰中毒抑制了以上所有检测因子,过量锰导致形态学的免疫损伤和炎症损伤。60天胸腺IL-7、60天脾脏NF-κB、30天的胸腺iNOS和30天脾脏PGE2的m RNA表达随着时间的增加而增加。2.IL-4、IL-7、IL-10和IL-17的关系较近,且与IFN-γm RNA表达成负相关关系。3.锰中毒导致了鸡法氏囊、胸腺和脾脏免疫损伤和炎性损伤。
[Abstract]:Manganese (Manganese,Mn) is a widely distributed element in nature. Manganese shows biological function in different links of life activities, which is directly related to enzyme synthesis, three major substance metabolism, hematopoiesis, immunity and reproductive function. Manganese is an essential nutrient element in organism, but excessive manganese is harmful to organism. Damage to the nervous, reproductive and immune systems caused by manganese has been confirmed. However, the effects of manganese on immune system cytokines and inflammatory factors in chickens are rarely studied. In this study, the effects of manganese poisoning on cytokines and inflammatory factors in immune organs of chickens were studied by establishing a model of manganese poisoning. 180 7-day-old sea blue egg roosters were divided into 4 groups and the control group was fed with standard diet. Three treatment groups were fed with standard diet supplemented with different concentrations of Mn Cl2 (feed manganese concentrations were 600900 and 1800 mg/kg, respectively). After manganese treatment for 30, 60 and 90 days, bursa of Fabricius, thymic gland and spleen were collected to detect the expression of IL-4,IL-7,IL-10,IL-17,TGF- 尾 4, IFN- 纬, NF- 魏 B, TNF- 伪, COX-2,PGE2 and iNOSm RNA in three immune organs. The difference of detection factors among different groups was analyzed by single factor variance analysis, and the relationship among cytokines was analyzed by multivariate correlation analysis, principal component analysis and cluster analysis. The results are as follows: 1.30 and 60 days manganese poisoning upregulated NO content, iNOS activity, IL-4,IL-7,IL-10,IL-17,TGF- 尾 4, NF- 魏 B, TNF- 伪, COX-2, in bursa of Fabricius, thymus and spleen. The expression of PGE2 and iNOS mRNA inhibited the expression of IFN- 纬 mRNA, manganese poisoning inhibited all the above factors on the 90th day, excessive manganese induced morphological immune injury and inflammatory injury. On the 60th day of thymic IL-7,60, spleen NF- kappa B, 2. Il-4, IL-7, IL-10 and IL-17 were closely related to the expression of m-RNA in thymus iNOS and 30-day spleen PGE2. 3. The expression of IL-4, IL-7, IL-10 and IL-17 was negatively correlated with the expression of IFN- 纬 m RNA. Manganese poisoning results in immune and inflammatory damage to bursa Fabricius thymus and spleen in chickens.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.31

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