日本血吸虫高迁移率族蛋白活化鼠巨噬细胞的初步研究
发布时间：2019-05-15 12:49
【摘要】：为研究日本血吸虫高迁移率族蛋白(Schistosoma japonicum high mobility group box1,Sj HMGB1)体外活化小鼠巨噬细胞的功能,利用真核重组Sj HMGB1蛋白与巨噬细胞共培养48 h,流式细胞术检测巨噬细胞表面标志分子以及趋化因子受体的表达。收集Sj HMGB1蛋白与巨噬细胞共孵育48 h后上清,ELISA检测上清中TNF-α与IL-10的含量,Griess法检测上清中NO的含量。流式结果显示,与对照组相比,Sj HMGB1蛋白刺激组的巨噬细胞表面MHC-Ⅱ分子,TLR4受体以及趋化因子受体CCR7的表达显著上调且差异具有极显著统计学意义(P0.01),TLR2受体的表达显著下调,差异具有显著统计学意义(P0.05)。ELISA结果显示,Sj HMGB1能够刺激巨噬细胞分泌致炎因子TNF-α,未能促进抑炎因子IL-10的释放。Griess法表明Sj HMGB1能够促进巨噬细胞分泌NO。本研究结果表明Sj HMGB1可能通过TLR4通路活化小鼠巨噬细胞并诱导其向致炎性M1型巨噬细胞极化。
[Abstract]:In order to study the activation of mouse macrophages with high mobility group protein (Schistosoma japonicum high mobility group box1,Sj HMGB1 of Schistosoma japonicum in vitro, the recombinant Sj HMGB1 protein was co-cultured with macrophages for 48 h. The expression of macrophage surface markers and chemokine receptors were detected by flow cytometry. After incubated with macrophages for 48 hours, Sj HMGB1 protein was collected. The contents of TNF- 伪 and IL-10 in the supernatant were detected by ELISA, and the content of NO in the supernatant was detected by Griess. The results of flow cytometry showed that the expression of MHC- 鈪，
本文编号：2477519
[Abstract]:In order to study the activation of mouse macrophages with high mobility group protein (Schistosoma japonicum high mobility group box1,Sj HMGB1 of Schistosoma japonicum in vitro, the recombinant Sj HMGB1 protein was co-cultured with macrophages for 48 h. The expression of macrophage surface markers and chemokine receptors were detected by flow cytometry. After incubated with macrophages for 48 hours, Sj HMGB1 protein was collected. The contents of TNF- 伪 and IL-10 in the supernatant were detected by ELISA, and the content of NO in the supernatant was detected by Griess. The results of flow cytometry showed that the expression of MHC- 鈪，
本文编号：2477519
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