陕西杨凌某蛋鸡场禽戊型肝炎病毒的检测
发布时间：2019-05-18 05:10
【摘要】：2014年-2015年陕西杨凌地区部分商品蛋鸡场产蛋量下降了10%~40%,剖检部分鸡只发现肝脏肿大出血。为确诊该疫情的病因,从发病的6个蛋鸡场采集了337份血清和268份粪便,利用间接ELISA和套式RT-PCR分别对血清中的禽戊型肝炎病毒(HEV)抗体和粪便中的禽HEV RNA进行了实验室检测。ELISA检测结果发现,168份血清为禽HEV抗体阳性,阳性率为49.85%;套式RT-PCR检测结果发现,63份粪便为禽HEV RNA基因片段阳性,阳性率为23.51%。基因片段的序列同源性分析发现,杨凌地区的禽HEV分离株与国内的同源性为93.0%~99.2%;进化树分析表明,其与国内其他地区的分离株在同一进化分支上,同属禽HEV基因3型。
[Abstract]:From 2014 to 2015, the egg production of some commercial laying hens in Yang Ling area of Shaanxi Province decreased by 10% to 40%. Some chickens were found to have massive liver bleeding. In order to confirm the cause of the epidemic, 337 serum and 268 faeces were collected from 6 laying hens. Indirect ELISA and nest RT-PCR were used to detect avian hepatitis E virus (HEV) antibody in serum and avian HEV RNA in feces, respectively. the results of Elisa showed that 168 serum samples were positive for avian HEV antibody, the positive rate was 49.85%. The results of nest RT-PCR showed that 63 feces were positive for avian HEV RNA gene fragment, the positive rate was 23.51%. The sequence homology analysis of the gene fragment showed that the homology between the avian HEV isolate in Yang Ling area and that in China was 93.0% 鈮，
本文编号：2479705
[Abstract]:From 2014 to 2015, the egg production of some commercial laying hens in Yang Ling area of Shaanxi Province decreased by 10% to 40%. Some chickens were found to have massive liver bleeding. In order to confirm the cause of the epidemic, 337 serum and 268 faeces were collected from 6 laying hens. Indirect ELISA and nest RT-PCR were used to detect avian hepatitis E virus (HEV) antibody in serum and avian HEV RNA in feces, respectively. the results of Elisa showed that 168 serum samples were positive for avian HEV antibody, the positive rate was 49.85%. The results of nest RT-PCR showed that 63 feces were positive for avian HEV RNA gene fragment, the positive rate was 23.51%. The sequence homology analysis of the gene fragment showed that the homology between the avian HEV isolate in Yang Ling area and that in China was 93.0% 鈮，
本文编号：2479705
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