LiCl对绒山羊毛囊生长和β-catenin表达的影响
发布时间：2019-05-19 08:16
【摘要】：为研究不同剂量LiCl对绒山羊毛囊生长和β-连环蛋白(β-catenin)m RNA表达的影响,将分离的绒山羊初级毛囊随机分为5组,每组24个重复,对照组为William'E基础培养液。试验组在基础培养液中依次添加2,5,10和20mmol·L~(-1)的LiCl,培养期为7d,每日测量毛囊生长长度。运用荧光定量PCR技术测定各组β-cateninm RNA表达水平。结果表明:2~10 mmol·L~(-1)LiCl组的β-catenin m RNA表达水平和生长长度极显著高于对照组(p0.01),毛囊形态维持良好;LiCl浓度为20mmol·L~(-1)组的β-catenin m RNA表达量及生长长度极显著低于对照组(p0.01),体外培养毛囊生长受到抑制。添加2~10mmol·L~(-1)的LiCl可促进绒山羊毛囊生长,促使β-catenin m RNA表达水平提高,其中5mmol·L~(-1)为较优浓度。通过本试验研究发现,适量的LiCl在一定程度上有利于初级毛囊的生长及形态的维持,促进了β-catenin m RNA表达。
[Abstract]:In order to study the effects of different doses of LiCl on hair follicles growth and 尾-catenin) m RNA expression of cashmere goats, the isolated primary hair follicles of cashmere goats were randomly divided into 5 groups with 24 repeats in each group, and the control group was the basic culture medium of William'E. The experimental group added 2, 5, 10 and 20mmol 路L ~ (- 1) LiCl, culture period to the basic culture medium in turn, and the hair follicles growth length was measured every day. The expression of 尾-cateninm RNA in each group was measured by fluorescence quantitative PCR. The results showed that the expression level and growth length of 尾-catenin m RNA in 2 鈮，
本文编号：2480557
[Abstract]:In order to study the effects of different doses of LiCl on hair follicles growth and 尾-catenin) m RNA expression of cashmere goats, the isolated primary hair follicles of cashmere goats were randomly divided into 5 groups with 24 repeats in each group, and the control group was the basic culture medium of William'E. The experimental group added 2, 5, 10 and 20mmol 路L ~ (- 1) LiCl, culture period to the basic culture medium in turn, and the hair follicles growth length was measured every day. The expression of 尾-cateninm RNA in each group was measured by fluorescence quantitative PCR. The results showed that the expression level and growth length of 尾-catenin m RNA in 2 鈮，
本文编号：2480557
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