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不同猪种泌乳期母乳外核体中let-7c表达模式研究

发布时间:2019-05-19 17:10
【摘要】:为了研究不同猪种泌乳期母乳外核体中let-7c的表达模式,试验采用荧光定量PCR(QPCR)方法检测了不同泌乳期(0,3,7,14,21,28天)的大约克夏猪和梅山猪母乳外核体中let-7c的发育性表达模式。结果表明:两个猪种不同泌乳阶段母乳外核体中let-7c的发育表达模式十分相似,总体趋势均表现为随时间推移而下降,且泌乳前期(0~3 d)免疫球蛋白(Ig E)的表达水平极显著高于泌乳后期(7~28 d)(P0.01);品种间分析结果显示,梅山猪母乳外核体中的let-7c表达水平在整个泌乳期均明显高于大约克夏猪。
[Abstract]:In order to study the expression pattern of let-7c in the outer nucleus of breast milk of different pig breeds during lactation, fluorescence quantitative PCR (QPCR) (FQ-PCR) was used to detect the expression patterns of VEGF in different lactation stages. 7, 14, 21, 28 days) the developmental expression pattern of let-7c in the outer milk nucleus of York pigs and Meishan pigs. The results showed that the developmental and expression patterns of let-7c in the outer nucleus of breast milk of the two breeds were very similar at different lactation stages, and the overall trend decreased with the passage of time. The expression level of immunoglobulin (Ig E) in the early lactation stage (0 鈮,

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