高脂日粮对初情期小鼠乳腺发育的影响及分子机制

发布时间：2019-05-28 19:26

【摘要】：【目的】研究高脂日粮对初情期小鼠乳腺发育的影响及可能机制。【方法】选用20只4周龄昆明雌性小鼠,设对照组和高脂日粮组,试验期4周,每周称体质量并统计采食量。试验结束后采集小鼠乳腺并称质量,采用wholemount及HE染色观察小鼠乳腺组织形态结构及导管数量。利用Western blot检测初情期小鼠乳腺组织胰岛素样生长因子1(Insulin-like growth factor1,IGF-1)、增殖相关信号通路关键蛋白蛋白激酶B(Protein kinase B,Akt)、胞外调控的蛋白激酶(Extracellular regulated protein kinase,Er K)以及炎症信号通路关键蛋白Toll样受体4(Toll-like receptor 4,TLR4)、c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)和核因子κB激酶抑制剂(Inhibitor of nuclear factor kappa-B kinase,IKK)的表达。【结果】高脂日粮组小鼠采食量极显著低于对照组(P0.01),但在能量摄入方面与对照组无显著差异。高脂日粮组小鼠的体质量增加显著高于对照组(P0.05)。高脂日粮组小鼠在乳腺质量、乳腺质量与体质量的比值上均极显著高于对照组(P0.01)。乳腺组织的whole-mount及HE染色结果表明,高脂日粮组小鼠乳腺导管发育明显被抑制,其乳腺组织的导管数和密度明显降低,乳腺组织的终末乳芽数量也极显著低于对照组(P0.01)。高脂日粮能够显著抑制小鼠乳腺组织IGF-I的蛋白表达以及Akt、Erk和IKK的磷酸化水平(P0.01或0.05),极显著促进小鼠乳腺组织TLR4的蛋白表达(P0.01)和显著促进JNK的磷酸化水平(P0.05)。【结论】高脂日粮能够抑制初情期小鼠乳腺发育,这可能是通过抑制乳腺增殖的相关信号和促进乳腺组织炎症的相关信号来实现。
[Abstract]:[objective] to study the effect of high fat diet on breast development of first estrus mice and its possible mechanism. [methods] Twenty 4-week-old Kunming female mice were divided into control group and high-fat diet group for 4 weeks. At the end of the experiment, the mammary gland mass of mice was collected, and the morphological structure of breast tissue and the number of ducts were observed by wholemount and HE staining. Insulin-like growth factor-1 (Insulin-like growth factor1,IGF-1), proliferation-related signaling pathway key protein kinases B (Protein kinase B, Akt) and extracellular regulated protein kinase (Extracellular regulated protein kinase, were detected by Western blot in breast tissue of estrous mice. Er K) and Toll-like receptor 4 (Toll-like receptor 4, TLR4), c-Jun N-terminal kinase (c-Jun N-terminal kinase,JNK) and (Inhibitor of nuclear factor kappa-B kinase, an inhibitor of nuclear factor kappa B kinase, are the key proteins of inflammatory signaling pathway. The expression of IKK). [results] the feed intake of mice in high fat diet group was significantly lower than that in control group (P 0.01), but there was no significant difference in energy intake between high fat diet group and control group. The body mass of mice in high fat diet group was significantly higher than that in control group (P 0.05). The breast mass and the ratio of breast mass to body mass in the high fat diet group were significantly higher than those in the control group (P 0.01). The results of whole-mount and HE staining in breast tissue showed that the development of mammary duct was significantly inhibited and the number and density of ducts in breast tissue were significantly decreased in the high fat diet group. The number of terminal buds in breast tissue was also significantly lower than that in the control group (P 0.01). High fat diet could significantly inhibit the expression of IGF-I protein and the phosphorylation of Akt,Erk and IKK in breast tissue of mice (P01 or 0.005). It significantly promoted the expression of TLR4 protein in breast tissue of mice (P 0.01) and the phosphorylation level of JNK (P 0.05). [conclusion] High fat diet can inhibit the development of mammary gland in mice during first estrus. This may be achieved by inhibiting the signal of breast proliferation and promoting inflammation of breast tissue.
【作者单位】：华南农业大学动物科学学院;
【基金】：国家重点研发计划(2016YFD0500503) 广东省特支计划(2014TQ01N260)
【分类号】：S816

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