呼和浩特市流浪犬狂犬病病毒血清学调查
发布时间:2019-05-28 19:06
【摘要】:为了解呼和浩特市流浪犬狂犬病病毒携带情况,笔者采用双抗夹心ELISA方法,检测采自呼和浩特市的58条流浪犬的血清,并进行小鼠颅内注射试验予以验证。结果表明:在所采集的58份血样中,检出阳性血清8份,阳性率为13.8%,小鼠验证试验中,注射阳性血清的8组小鼠中,其中7组动物均表现出抽搐、食欲下降等症状,饲养7~12 d内死亡;另有1个低浓度剂量组未见明显临床症状。可见呼和浩特市流浪犬狂犬病带毒率较高。
[Abstract]:In order to understand the carrying situation of rabies virus in stray dogs in Hohhot, the serum of 58 stray dogs collected from Hohhot was detected by double antibody sandwich ELISA and verified by intracranial injection test in mice. The results showed that 8 of the 58 blood samples were positive, the positive rate was 13.8%. In the verification test of mice, 7 of the 8 groups of mice injected with positive serum showed symptoms such as convulsions and loss of appetite. Died within 12 days of feeding. In another low concentration dose group, no obvious clinical symptoms were found. It can be seen that the rabies carrying rate of stray dogs in Hohhot is high.
【作者单位】: 农业部动物疾病临床诊疗技术重点实验室/内蒙古农业大学兽医学院;
【基金】:内蒙古自治区研究生教育创新计划研究生科研创新资助项目(2015)
【分类号】:S858.292
[Abstract]:In order to understand the carrying situation of rabies virus in stray dogs in Hohhot, the serum of 58 stray dogs collected from Hohhot was detected by double antibody sandwich ELISA and verified by intracranial injection test in mice. The results showed that 8 of the 58 blood samples were positive, the positive rate was 13.8%. In the verification test of mice, 7 of the 8 groups of mice injected with positive serum showed symptoms such as convulsions and loss of appetite. Died within 12 days of feeding. In another low concentration dose group, no obvious clinical symptoms were found. It can be seen that the rabies carrying rate of stray dogs in Hohhot is high.
【作者单位】: 农业部动物疾病临床诊疗技术重点实验室/内蒙古农业大学兽医学院;
【基金】:内蒙古自治区研究生教育创新计划研究生科研创新资助项目(2015)
【分类号】:S858.292
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