一种缓释FSH注射剂对奶牛发情和超排的效果观察
发布时间：2019-06-10 06:26
【摘要】：奶牛的繁殖效率和优良奶牛繁殖性能的提高直接影响奶牛业的效益。在繁殖机能正常的母牛中,随着卵泡的生长发育和退化的交替进行,自然条件下往往只有一个优势卵泡发育成熟排出。因此,如何在日常工作中,既不影响健康奶牛正常生产性能,又可大幅度的提高优质母牛的繁殖性能一直是奶牛养殖业关注的重点。超数排卵和胚胎移植技术作为解决这一问题的最有效途径和方法得到越来越多的重视。FSH作为诱导发情和超数排卵技术中的关键药物,其质量和正确使用则直接影响诱导发情和超排的效果。为此,本试验重点观察了一种新型的FSH缓释注射剂对奶牛诱导发情和超数排卵的效果,为该剂型的改进及其在奶牛场的应用提供实验依据。选择了15至18月龄的青年育成荷斯坦奶牛12头,随机分成两组各6头,试验组的处理:在奶牛发情周期的任意一天(非发情当天)进行超排处理,放置CIDR(当天记为第0 d),伴随肌肉注射4 mg E2,第10 d上午6:00一次性肌肉注射一定剂量的FSH缓释注射剂,第12 d上午和下午各肌肉注射25 mg PGF2α,第13 d下午撤除CIDR,第15 d观察供体母牛的发情表现(间隔),发情后12 h输精1次,间隔12 h进行第2次输精,第22 d(发情后第7 d)进行非手术采集胚胎。对照组的处理:除了注射FSH的方案与试验组不同之外其他的都相同,FSH注射方案:第10 d早上6:00注射2.0 mg FSH,下午6:00注射1.5 mg FSH;第11 d早上和晚上分别注射1.5 mg FSH;第12 d早上6:00注射FSH(1.0 mg)+PGF2α(25 mg);晚上注射FSH(0.5 mg)+PGF2α(25 mg)。结果表明,对照组发情率为100%,试验组发情率为83.33%,差异不显著(p0.05)对照组奶牛的可用胚胎数平均为3.16枚/头,黄体数为6.83个/头,胚胎有效率为65.52%;试验组奶牛的可用胚胎数平均为2.0枚/头,黄体数为2.80个/头,胚胎有效率为66.67%。试验组与对照组黄体数相比差异显著(p0.05)。结论:缓释FSH注射剂对荷斯坦黑白花奶牛的超数排卵具有一定的效果。
[Abstract]:The improvement of breeding efficiency and excellent reproductive performance of dairy cows directly affects the benefit of dairy industry. In cows with normal reproductive function, with the alternation of follicular growth, development and degeneration, there is often only one dominant follicular development and maturation under natural conditions. Therefore, how to not affect the normal performance of healthy cows in daily work, but also greatly improve the reproductive performance of high-quality cows has always been the focus of attention of dairy cattle breeding industry. As the most effective way and method to solve this problem, superovulatory and embryo transfer techniques have been paid more and more attention. As the key drug in estrus induction and superovulatory technology, FSH has been used as the key drug in estrus induction and superovulatory technology. Its quality and correct use directly affect the effect of estrus induction and superexcretion. Therefore, the effect of a new type of FSH sustained release injection on estrus and superovulatory induction in dairy cows was observed, which provided experimental basis for the improvement of the dosage form and its application in dairy farms. Twelve Holstein cows aged 15 to 18 months were randomly divided into two groups. The experimental group was treated with superexcretion on any day of estrus cycle (non-estrus day) and placed CIDR (0 d), on the same day). With intramuscular injection of 4 mg E2, a certain dose of FSH sustained release injection was injected intramuscular at 6:00 on the 10th day, 25 mg PGF2 伪 was injected intramuscular in the morning and afternoon on the 12th day, and CIDR, was removed in the afternoon of the 13th day. The estrus performance of donor cows was observed on the 15th day. The ejaculation was performed at 12 hours after estrus, the second time at 12 hours after estrus, and the embryos were collected by non-operation on the 22nd day (7 days after estrus). Treatment of the control group: except that the regimen of FSH injection was the same as that of the experimental group, FSH injection regimen: injection of 2.0 mg FSH, at 6:00 on the 10th day, 1.5 mg FSH; at 6:00 Injection of FSH (1.0 mg) PGF2 伪 (25 mg);) FSH (0.5 mg) PGF2 伪 (25 mg).) at 6:00 on the 12th day after injection of 1.5 mg in the morning and evening on the 11th day, respectively. The results showed that the estrus rate of the control group was 100%, and the estrus rate of the experimental group was 83.33%. The difference was not significant (p0.05). The average number of available embryos and corpus of the control group were 3.16 / head and 6.83 / head, respectively. The effective rate of embryos was 65.52%. In the experimental group, the average number of available embryos was 2.0 / head, the number of corpus was 2.80 / head, and the effective rate of embryos was 66.67%. There was significant difference in corpus number between the experimental group and the control group (p0.05). Conclusion: sustained release FSH injection has certain effect on superovulatory of Holstein black and white dairy cows.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823.3
本文编号：2496247
[Abstract]:The improvement of breeding efficiency and excellent reproductive performance of dairy cows directly affects the benefit of dairy industry. In cows with normal reproductive function, with the alternation of follicular growth, development and degeneration, there is often only one dominant follicular development and maturation under natural conditions. Therefore, how to not affect the normal performance of healthy cows in daily work, but also greatly improve the reproductive performance of high-quality cows has always been the focus of attention of dairy cattle breeding industry. As the most effective way and method to solve this problem, superovulatory and embryo transfer techniques have been paid more and more attention. As the key drug in estrus induction and superovulatory technology, FSH has been used as the key drug in estrus induction and superovulatory technology. Its quality and correct use directly affect the effect of estrus induction and superexcretion. Therefore, the effect of a new type of FSH sustained release injection on estrus and superovulatory induction in dairy cows was observed, which provided experimental basis for the improvement of the dosage form and its application in dairy farms. Twelve Holstein cows aged 15 to 18 months were randomly divided into two groups. The experimental group was treated with superexcretion on any day of estrus cycle (non-estrus day) and placed CIDR (0 d), on the same day). With intramuscular injection of 4 mg E2, a certain dose of FSH sustained release injection was injected intramuscular at 6:00 on the 10th day, 25 mg PGF2 伪 was injected intramuscular in the morning and afternoon on the 12th day, and CIDR, was removed in the afternoon of the 13th day. The estrus performance of donor cows was observed on the 15th day. The ejaculation was performed at 12 hours after estrus, the second time at 12 hours after estrus, and the embryos were collected by non-operation on the 22nd day (7 days after estrus). Treatment of the control group: except that the regimen of FSH injection was the same as that of the experimental group, FSH injection regimen: injection of 2.0 mg FSH, at 6:00 on the 10th day, 1.5 mg FSH; at 6:00 Injection of FSH (1.0 mg) PGF2 伪 (25 mg);) FSH (0.5 mg) PGF2 伪 (25 mg).) at 6:00 on the 12th day after injection of 1.5 mg in the morning and evening on the 11th day, respectively. The results showed that the estrus rate of the control group was 100%, and the estrus rate of the experimental group was 83.33%. The difference was not significant (p0.05). The average number of available embryos and corpus of the control group were 3.16 / head and 6.83 / head, respectively. The effective rate of embryos was 65.52%. In the experimental group, the average number of available embryos was 2.0 / head, the number of corpus was 2.80 / head, and the effective rate of embryos was 66.67%. There was significant difference in corpus number between the experimental group and the control group (p0.05). Conclusion: sustained release FSH injection has certain effect on superovulatory of Holstein black and white dairy cows.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823.3
【参考文献】
相关期刊论文 前1条
1 付静涛,吴通义,余文莉,李树静;放牧条件下安格斯、海福特肉牛重复超排的研究[J];中国畜牧杂志;2002年05期
，本文编号：2496247
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