细粒棘球绦虫原头蚴对小鼠巨噬细胞RAW264.7一氧化氮分泌的影响

发布时间：2019-06-10 19:34

【摘要】：分别用细粒棘球绦虫原头蚴(PSC)、包囊壁(HC)和包囊液(HF)刺激培养在24孔板上的小鼠巨噬细胞RAW 264.7,并用PBS作为对照,用G riess试剂检测不同时间点一氧化氮(NO)的浓度。结果显示,在短时期(48 h)内,PSC刺激巨噬细胞后在第8、24和48小时均产生大量NO,与PBS对照组相比均差异显著(P0.05);HC也可刺激巨噬细胞产生NO,与PBS对照组相比,在刺激后第24和48小时也均差异显著(P0.05);HF在刺激后第4、24和48小时均产生少量NO,但与PBS对照组相比均差异不显著(P0.05)。结果表明,巨噬细胞可通过不断释放大量NO来抵御原头蚴的侵染,包囊对宿主具有损害作用,同时又能通过降低NO的产生来减弱宿主对原头蚴的杀伤作用,包囊液对巨噬细胞释放NO的作用不显著。本研究为进一步揭示宿主在抵抗寄生虫感染时NO的作用机制奠定了基础。
[Abstract]:Mouse macrophage RAW 264.7 cultured on 24 well plate was stimulated by (PSC), cyst wall (HC) and capsule fluid (HF), respectively, and PBS was used as control. The concentration of nitric oxide (NO) at different time points was detected by G riess reagent. The results showed that in a short period of time (48 h), a large number of NO, were produced at the 8th, 24th and 48th hours after PSC stimulation of macrophages, which were significantly different from those in the PBS control group (P 0.05). HC could also stimulate macrophages to produce NO, compared with PBS control group, and there were significant differences at 24 and 48 hours after stimulation (P 0.05). HF produced a small amount of NO, at 4,24 and 48 hours after stimulation, but there was no significant difference compared with PBS control group (P 0.05). The results showed that macrophages could resist the infection of protocephalus by releasing a large amount of NO, and the capsule could damage the host, and at the same time, it could weaken the killing effect of the host to the protocephalus by reducing the production of NO. The effect of capsule solution on the release of NO from macrophages was not significant. This study laid a foundation for further revealing the mechanism of NO in host resistance to parasite infection.
【作者单位】：石河子大学动物科技学院;省部共建绵羊遗传改良与健康养殖国家重点实验室新疆农垦科学院新疆兵团绵羊繁育生物技术重点实验室;中国农业大学动物医学院;
【基金】：国家重点基础研究发展计划(973)项目(2015CB150300) 国家自然科学基金项目(31360608) 新疆生产建设兵团科技攻关项目(2014BA002)
【分类号】：S852.7

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