一株抑菌型地衣芽孢杆菌的快速分离与鉴定及其对4株耐药致病菌的抑制作用

发布时间：2019-06-19 11:32

【摘要】：[目的]本文旨在分离具有良好抗菌活性的芽孢杆菌菌株作为抗生素替代品材料。[方法]通过热处理和抑菌板双重筛选对革兰氏阳性菌和阴性菌均具有抗菌活性的芽孢杆菌,并通过形态学、生理生化和分子生物学方法鉴定分离菌株。体外测定分离菌株发酵上清液对4株耐药致病菌的抑制作用。[结果]分离到6株对革兰氏阳性菌和阴性菌均具有抗菌活性的芽孢杆菌,其中分离菌株BB11的抑菌活性最强,对沙门氏菌C782和大肠杆菌K_(12)D_(31)的抑菌圈直径分别达15.34和6.26 mm。染色镜检BB11菌为带芽孢的革兰氏阳性菌,理化特性与芽孢杆菌相符。BB11菌的16S rRNA序列与地衣芽孢杆菌(Bacillus licheniformis)BCRC 15413序列的相似度在99%以上,gyr B基因序列与B.licheniformis BCRC 12826序列的相似度达到100%。BB11菌株的发酵上清液对具有多重耐药的致病性大肠杆菌K88、K99、987P和沙门氏菌C782均表现出较好的抑杀作用。[结论]分离到的地衣芽孢杆菌BB11具有用于开发多功能微生态制剂的潜能。
[Abstract]:[Objective] The purpose of this paper is to isolate a Bacillus strain with good antibacterial activity as an antibiotic substitute material. [Methods] The bacteria of Gram-positive and negative-negative bacteria were screened by double screening of heat treatment and bacteriostatic plate, and isolated strains were identified by morphological, physiological and molecular biological methods. The inhibitory effect of the supernatant of the isolated strain on the 4 strains of drug-resistant pathogenic bacteria was determined in vitro. [Results] Six strains of bacillus with antibacterial activity against gram-positive and negative-negative bacteria were isolated, and the antibacterial activity of the isolated strain BB11 was the strongest, and the diameters of the antibacterial rings of the Salmonella C782 and the E. coli K _ (12) D _ (31) were 15.34 and 6.26 mm, respectively. The bacteria of BB11 were the Gram-positive bacteria with spore and the physical and chemical properties were in line with that of Bacillus. the similarity of the 16S rRNA sequence of the BB11 strain to the BCRC 15413 sequence of the Bacillus licheniformis BCRC 15413 is over 99%, and the similarity of the gyr B gene sequence to the B. lichleniformis BCRC 12826 sequence is up to 100%. The fermentation supernatant of the BB11 strain is a pathogenic E. coli K88 with multiple drug resistance, K99,987 P and Salmonella C782 showed a good anti-killing effect. [Conclusion] The isolated Bacillus licheniformis BB11 has the potential to develop a multifunctional microecological preparation.
【作者单位】：南京农业大学动物医学院;上海市奶牛研究所;上海市农业科学院上海种猪工程技术研究中心;
【基金】：上海市产业技术创新战略联盟专项(13DZ0510900) 上海工程技术研究中心建设专项(16dz2250700)
【分类号】：S816.7

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