某公司猪群主要疫病的感染情况调查
发布时间：2019-06-20 21:47
【摘要】：随着规模养猪业的发展,规模化养猪场的疫病种类和数量均有增加的趋势,从而对养猪业造成巨大的损失。为了了解规模化猪场中主要疫病的感染情况,本人采用PCR和RT-PCR方法,结合细菌分离鉴定,2014年8月至2015年3月,对某集团公司下属分别来自福建、广西、江西、上海、安徽5个省(市)的14个规模化猪场的126份组织样品(发病猪的脑、肺脏、脾脏、肾脏、心脏、淋巴结、关节积液等)分别进行了8种病原检测。结果显示:14个规模化猪场,86份样品均有不同病原的感染,猪场和组织样品的阳性率分别为100%(14/14)和68.25%(86/126)。其中,单独或混合感染猪圆环病毒2型(PCV2)、猪繁殖与呼吸综合征病毒(PRRSV)、猪瘟病毒(CSFV)、猪伪狂犬病毒(PRV)、猪肺炎支原体(Mhp)、猪传染性胸膜肺炎放线杆菌(APP)、猪链球菌(SS)、副猪嗜血杆菌(HPS)的阳性样品分别为50.79%(64/126)、24.6%(21/126)、7.14%(9/126)、0(0/126)、16.67%(21/126)、8.73%(11/126)、11.11%(14/126)、7.14%(9/126)。从感染的类型看,在86份阳性样品中,单独感染的样品31份,占36.05%(31/86);混合感染的样品55份,占63.95%(55/86)。从混合感染的类型看,在55份混合感染阳性样品中,二重混合感染39份、三重混合感染15份、四重混合感染1份,分别占70.91%(39/55)、27.27%(15/55)和1.82%(1/55)。从混合感染的微生物种类看,在55份混合感染阳性样品中,“病毒与病毒”混合感染的样品19份、“病毒与细菌”混合感染的样品36份,分别占34.55%(19/55)和65.45%(36/55)。从感染的优势病原分析,PCV2和/或PRRSV感染的样品75份,占86份阳性样品的87.21%。其中,“PCV2+PRRSV(+其它病原)”混合感染的样品20份,占所有55份混合感染样品的36.36%。结果表明:该集团公司下属规模猪场普遍存在病毒与细菌感染;感染类型复杂多样,混合感染相当普遍,以“PCV2十PRRSV(+其它病原)”混合感染最为常见。对分离得到的11株APP、14株SS、9株HPS进行药物敏感性试验。结果表明3种分离菌对14种抗菌素的敏感性不同,其中SS与HPS对头孢噻肟与头孢曲松钠敏感度极高,而APP对恩诺沙星敏感性极高。
[Abstract]:With the development of large-scale pig industry, the species and quantity of epidemic diseases in large-scale pig farms have an increasing trend, which causes great losses to pig industry. In order to understand the infection of the main epidemic diseases in large-scale pig farms, I used PCR and RT-PCR methods, combined with bacterial isolation and identification. From August 2014 to March 2015, 126 tissue samples (brain, lung, spleen, kidney, heart, lymph nodes) from 14 large-scale pig farms in Fujian, Guangxi, Jiangxi, Shanghai and Anhui provinces (cities) were collected from 14 large-scale pig farms in Fujian, Guangxi, Jiangxi, Shanghai and Anhui provinces (including brain, lung, spleen, kidney, heart and lymph nodes). Eight kinds of pathogens were detected respectively. The results showed that 86 samples from 14 large-scale pig farms were infected with different pathogens. The positive rates of pig farms and tissue samples were 100% (14 鈮，
本文编号：2503531
[Abstract]:With the development of large-scale pig industry, the species and quantity of epidemic diseases in large-scale pig farms have an increasing trend, which causes great losses to pig industry. In order to understand the infection of the main epidemic diseases in large-scale pig farms, I used PCR and RT-PCR methods, combined with bacterial isolation and identification. From August 2014 to March 2015, 126 tissue samples (brain, lung, spleen, kidney, heart, lymph nodes) from 14 large-scale pig farms in Fujian, Guangxi, Jiangxi, Shanghai and Anhui provinces (cities) were collected from 14 large-scale pig farms in Fujian, Guangxi, Jiangxi, Shanghai and Anhui provinces (including brain, lung, spleen, kidney, heart and lymph nodes). Eight kinds of pathogens were detected respectively. The results showed that 86 samples from 14 large-scale pig farms were infected with different pathogens. The positive rates of pig farms and tissue samples were 100% (14 鈮，
本文编号：2503531
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