布氏杆菌S2疫苗免疫绵羊血清抗体消长规律研究

发布时间：2019-06-26 21:20

【摘要】：为了解绵羊对布氏杆菌病S2疫苗免疫后的抗体消长规律,通过口服和注射2种方法对绵羊进行了布氏杆菌病S2疫苗免疫试验,利用虎红平板凝集试验(RBT)和试管凝集试验(SAT)以及c ELISA检测方法进行了血清抗体检测。结果表明:正常剂量口服免疫S2疫苗20 d后,RBT、SAT、c ELISA抗体阳性率均达到最高,分别是80%、70%、60%,并且在免疫100 d后羊血清抗体阳性率分别为RBT 20%、SAT 10%、c ELISA 10%,免疫250 d后3种方法检测结果均转为阴性。2倍剂量口服免疫S2疫苗20 d后,RBT、SAT、c ELISA均达到最高为60%,并在免疫100 d后均转为阴性。以正常剂量注射免疫S2疫苗免疫7 d后RBT和SAT抗体阳性率达到最高为80%,c ELISA抗体阳性率达到60%,30 d后达到80%,而疫苗免疫370 d后,RBT和SAT检测抗体阳性率下降到60%,c ELISA检测抗体阳性率依然80%;而2倍剂量注射免疫S2疫苗370 d后,RBT和c ELISA检测抗体阳性率仍保持着100%,SAT检测抗体阳性率达80%。结果表明,从免疫羊抗体产生及体内消长规律来看,布氏杆菌S2疫苗注射免疫明显优于口服免疫效果,且两种免疫途径的试验羊均且未发现明显接种副反应。
[Abstract]:In order to understand the regularity of antibody growth and decline of sheep immunized with brucellosis S2 vaccine, the immunization test of brucellosis S2 vaccine was carried out by oral administration and injection, and the serum antibody was detected by tiger red plate agglutination test (RBT), test tube agglutination test (SAT) and c ELISA assay. The results showed that the positive rate of RBT,SAT,c ELISA antibody was the highest after 20 days of oral immunization with S2 vaccine, 80%, 70% and 60%, respectively, and the positive rate of antibody in sheep serum was 20%, SAT 10%, c ELISA 10%, respectively. After immunization with 2 times oral immunization with S2 vaccine for 20 days, the positive rate of RBT,SAT,c ELISA reached the highest 60%. After 100 days of immunization, they all turned negative. After immunization with normal dose S2 vaccine for 7 days, the positive rate of RBT and SAT antibody reached the highest 80%, the positive rate of c ELISA antibody reached 60%, and reached 80% after 30 days of immunization, while the positive rate of RBT and SAT antibody decreased to 60% after 370d immunization, and the positive rate of c ELISA antibody was still 80%. However, after immunization with S2 vaccine at twice dose for 370 days, the positive rate of antibody detected by RBT and c-ELISA remained 100%, and the positive rate of antibody detected by SAT was 80%. The results showed that the immunization effect of brucellosis S2 vaccine was significantly better than that of oral immunization, and no obvious side effects were found in the sheep immunized with the two immune pathways.
【作者单位】：内蒙古农业大学兽医学院农业部动物临床诊疗技术重点实验室;
【基金】：内蒙古自治区科技计划项目(201502071)
【分类号】：S858.26

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