HPLC测定动物源性食品中倍他米松的残留
发布时间：2019-07-01 15:27
【摘要】：为建立检测动物性食品中倍他米松残留的高效液相色谱法。样品经乙酸乙酯提取后,进行液相色谱分析。采用Agilent C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇:水(60∶40,v∶v),流速为0.8mL/min,检测波长为240nm,柱温为30℃。方法的检出限和定量限分别为0.152μg/kg和0.502μg/kg。倍他米松在0.5~32μg/mL范围内线性关系良好,相关系数为0.998 9;选取5种动物源性食品进行倍他米松的加标回收试验,回收率在72.73%~93.06%之间,精密度RSD为3.78%(n=6)。本方法简便、可靠、重复性好,可用于动物性食品中倍他米松残留的检测。
[Abstract]:In ord to establish high performance liquid chromatography for detecting that residual of betamethasone in animal food. The sample was extracted with ethyl acetate and analyzed by liquid chromatography. An Agilent C18 (250 mm, 4.6 mm,5. mu.m) column was used, and the mobile phase was methanol: water (60:40, v: v), the flow rate was 0.8 mL/ min, the detection wavelength was 240 nm, and the column temperature was 30 鈩，
本文编号：2508600
[Abstract]:In ord to establish high performance liquid chromatography for detecting that residual of betamethasone in animal food. The sample was extracted with ethyl acetate and analyzed by liquid chromatography. An Agilent C18 (250 mm, 4.6 mm,5. mu.m) column was used, and the mobile phase was methanol: water (60:40, v: v), the flow rate was 0.8 mL/ min, the detection wavelength was 240 nm, and the column temperature was 30 鈩，
本文编号：2508600
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