酿酒葡萄皮渣对单栏饲养方式下公绵羊繁殖性能及睾丸抗氧化性的影响
发布时间：2019-07-16 08:10
【摘要】：旨在研究日粮中添加一定量的酿酒葡萄皮渣对绵羊繁殖性能及睾丸抗氧化性能的影响。试验选取30只5月龄,体重(25±1)kg的杜泊×小尾寒羊杂交公羊,采用完全随机设计平均分为5组。1组自由活动,其余4组单栏饲养,建立应激模型。自由活动绵羊饲喂未添加葡萄皮渣日粮,单栏饲养绵羊分别饲喂不同葡萄皮渣添加水平(0%、5%、10%、20%)日粮。试验期80d。试验结束后,屠宰取样,测定睾丸系数并作附睾精子分析,测定睾丸组织抗氧化水平,对睾丸组织中Cu-ZnSOD、CAT、GPx4及Nrf2mRNA和蛋白相对表达量进行研究。结果表明,与自由活动组相比,单栏组绵羊睾丸组织中MDA及ROS水平升高(P0.01),睾丸重量显著降低(P0.05),睾丸系数也表现出下降趋势(P=0.06),附睾精子密度及顶体完整率显著降低(P0.05),精子活动率极显著降低(P0.01),精子畸形率极显著升高(P0.01),表明单栏饲养方式对绵羊造成氧化应激,并在一定程度上影响绵羊繁殖性能。适量添加酿酒葡萄皮渣后,绵羊附睾精子密度和精子活动率均显著升高(P0.05),精子畸形率显著降低(P0.05),睾丸组织MDA含量显著下降(P0.01),SOD、CAT及GSH-Px活性均显著提高(P0.01,P0.05,P0.01);睾丸组织中Cu-ZnSOD mRNA相对表达量显著提高(P0.05),Cu-ZnSOD、CAT、GPx4蛋白相对表达量显著提高(P0.05)。综上表明,酿酒葡萄皮渣可以提高单栏饲养方式下绵羊睾丸组织抗氧化性,进而改善绵羊繁殖性能。
[Abstract]:The purpose of this study was to study the effect of adding a certain amount of wine grape skin residue on the reproductive performance and testicular antioxidant performance of sheep. Thirty 5-month-old Dupo 脳 small-tailed Han sheep crossbred rams weighing (25 卤1) kg were randomly divided into 5 groups. Group 1 was free to move, and the other four groups were fed in a single column to establish stress model. The free moving sheep were fed with diet without grape skin residue, and the sheep fed with different levels of grape skin residue (0%, 5%, 10%, 20%) were fed with different levels of grape skin residue (0%, 5%, 10%, 20%). The experimental period is 80 days. At the end of the experiment, the testicular coefficient was measured and the epididymal sperm was analyzed. The antioxidant level of testicular tissue was measured, and the relative expression of Cu-ZnSOD,CAT,GPx4, Nrf2mRNA and protein in testicular tissue was studied. The results showed that compared with the free movement group, the levels of MDA and ROS in the testicular tissue of sheep in the single column group were increased (P01), the testicular weight was significantly decreased (P05), the testicular coefficient was also decreased (P 鈮，
本文编号：2514951
[Abstract]:The purpose of this study was to study the effect of adding a certain amount of wine grape skin residue on the reproductive performance and testicular antioxidant performance of sheep. Thirty 5-month-old Dupo 脳 small-tailed Han sheep crossbred rams weighing (25 卤1) kg were randomly divided into 5 groups. Group 1 was free to move, and the other four groups were fed in a single column to establish stress model. The free moving sheep were fed with diet without grape skin residue, and the sheep fed with different levels of grape skin residue (0%, 5%, 10%, 20%) were fed with different levels of grape skin residue (0%, 5%, 10%, 20%). The experimental period is 80 days. At the end of the experiment, the testicular coefficient was measured and the epididymal sperm was analyzed. The antioxidant level of testicular tissue was measured, and the relative expression of Cu-ZnSOD,CAT,GPx4, Nrf2mRNA and protein in testicular tissue was studied. The results showed that compared with the free movement group, the levels of MDA and ROS in the testicular tissue of sheep in the single column group were increased (P01), the testicular weight was significantly decreased (P05), the testicular coefficient was also decreased (P 鈮，
本文编号：2514951
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