解淀粉芽孢杆菌对免疫抑制肉鸡生长性能和肝脏抗氧化功能的影响

发布时间：2019-07-19 14:09

【摘要】：旨在研究解淀粉芽孢杆菌(Bacillus amyloliquefaciens,BA)对环磷酰胺(cyclophosphamide,CY)介导的免疫抑制肉鸡生长性能和肝脏抗氧化功能的影响。144只1日龄AA雄性肉仔鸡被随机分为3组,即对照组(CON),环磷酰胺处理组(CY)和环磷酰胺-解淀粉芽孢杆菌组(CY-BA),每组6个重复,每个重复8只鸡。CON组和CY组饲喂基础日粮,CY-BA组饲喂添加1.08×1010 CFU/kg BA的试验日粮。于16,17和18日龄对CY组与CY-BA组肉鸡肌肉注射80mg/kg的CY,CON组肌肉注射等量生理盐水,试验期为21d。结果显示,与CON组相比,CY刺激显著降低了CY组肉鸡1~21日龄体增质量、肝脏指数、肝脏总抗氧化能力(total antioxidative capacity,T-AOC)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力和谷胱甘肽还原酶(glutathione reductase,GR)活力(P0.05),显著升高了肝脏丙二醛(malondialdehyde,MDA)含量(P0.05);Real-time PCR检测也发现,CY处理显著降低了CY组肉鸡肝脏核转录相关因子2(NF-E2-related factor 2,nrf2)和谷胱甘肽过氧化物酶1(glutathione peroxidase 1,gpx1)的mRNA表达水平(P0.05)。与CY组相比,日粮添加BA显著提高了CY-BA组肉鸡21日龄时肝脏T-AOC水平和GSH-Px活力,降低了肝脏MDA水平(P0.05)。上述指标在CON与CY-BA组间并无明显差异(P0.05)。另外,CY-BA组肝脏GSH含量较CON或CY组均显著升高(P0.05)。然而,日粮添加BA并未显著影响CY刺激肉鸡体增质量、肝脏指数、肝脏nrf2和gpx1mRNA水平(P0.05)。综合结果表明,CY诱导的免疫抑制损伤了肉鸡生长和肝脏的抗氧化功能,并引起了肉鸡肝脏脂质过氧化程度升高。日粮添加BA具有改善CY介导的免疫抑制肉鸡肝脏抗氧化功能和缓解肝脏氧化损伤的作用。
[Abstract]:The purpose of this study was to study the effects of Bacillus amyloliquefaciens,BA on the growth performance and liver antioxidant function of immunosuppressive broilers mediated by cyclophosphamide (cyclophosphamide,CY). 144 1-day-old AA male broilers were randomly divided into three groups: control group (CON), cyclophosphamide treatment group (CY) and cyclophosphamide-starch-hydrolyzed spores group (CY-BA), with 6 repeats in each group. The basal diet was fed in CON group and CY group, and the experimental diet supplemented with 1.08 脳 1010 CFU/kg BA was fed in CY-BA group. At the age of 16, 17 and 18 days, the broilers in CY group and CY-BA group were injected with 80mg/kg intramuscular injection of the same amount of normal saline, the experimental period was 21 days. The results showed that compared with CON group, CY stimulation significantly decreased the weight gain, liver index, total antioxidant capacity (total antioxidative capacity,T-AOC, glutathione peroxidase,GSH-Px activity and glutathione reductase,GR activity of broilers in CY group (P 0.05), and significantly increased the content of malondialdehyde (malondialdehyde,MDA) in liver (P 0.05). Real-time PCR assay also showed that CY treatment significantly decreased the mRNA expression of nuclear transcription related factor 2 (NF-E2-related factor 2, nrf2) and glutathione peroxide 1 (glutathione peroxidase 1, gpx1 in CY group (P 0.05), and decreased the expression of nuclear transcription related factor 2 (nrf2, nrf2) and glutathione peroxide 1 (Gpx1) in liver of broilers in CY group (P 0.05). Compared with CY group, dietary BA significantly increased T-AOC level and GSH-Px activity in liver and decreased liver MDA level in CY-BA group at 21 days of age (P 0.05). There was no significant difference between CON and CY-BA (P 0.05). In addition, the content of GSH in liver of CY-BA group was significantly higher than that of CON or CY group (P 0.05). However, dietary BA supplementation did not significantly affect body weight gain, liver index, liver nrf2 and gpx1mRNA levels in broilers stimulated by CY (P 0.05). The results showed that immunosuppression induced by CY damaged the growth of broilers and the antioxidant function of liver, and increased the degree of lipid peroxide in liver of broilers. Dietary addition of BA can improve the antioxidant function of liver mediated by CY and alleviate the oxidative damage of liver in broilers.
【作者单位】：南京农业大学动物科技学院南京农业大学食品科技学院
【基金】：“十二五”国家科技支撑计划资助项目(2013BAD10B02-03)
【分类号】：S831.5

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