表皮生长因子对葡聚糖硫酸钠诱导的结肠炎模型小鼠肠道损伤的修复研究

发布时间：2019-07-19 12:32

【摘要】：本文旨在讨论表皮生长因子(EGF)对葡聚糖硫酸钠(DSS)诱导的结肠炎模型小鼠肠道损伤的修复作用。选用24只6周龄BALB/c小鼠,随机分为3组,即:正常对照组、DSS模型对照组、DSS+EGF组。正常对照组小鼠饮用自来水;DSS模型对照组小鼠在试验第1~7天饮用5%DSS水溶液,第8~10天饮用自来水;DSS+EGF组小鼠按照DSS模型对照组处理,同时每天皮下注射EGF 2次,共注射10 d。结果表明:1)与正常对照组相比,DSS模型对照组小鼠结肠长度极显著降低(P0.01);与DSS模型对照组相比,DSS+EGF组小鼠结肠长度极显著增加(P0.01)。2)DSS模型对照组小鼠结肠可见典型溃疡,结肠损伤程度评分(CDS)极显著高于正常对照组(P0.01);DSS+EGF组小鼠结肠组织未见溃疡,与DSS模型对照组相比,CDS极显著降低(P0.01)。3)与正常对照组相比,DSS模型对照组小鼠结肠紧密连接蛋白(Occludin)浓度显著降低(P0.05);与DSS模型对照组相比,DSS+EGF组小鼠结肠Occludin浓度显著升高(P0.05)。4)与正常对照组相比,DSS模型对照组小鼠结肠白细胞介素-2(IL-2)和白细胞介素-4(IL-4)浓度极显著降低(P0.01),白细胞介素-10(IL-10)浓度显著降低(P0.05);与DSS模型对照组相比,DSS+EGF组结肠IL-2和IL-4浓度极显著增加(P0.01),IL-10浓度显著增加(P0.05)。由此可见,EGF可能通过提高肠道Occludin表达水平,调节肠道细胞因子浓度趋于正常水平,从而修复受损肠道组织,维持肠道黏膜屏障的完整性。
[Abstract]:The purpose of this study was to investigate the repair effect of epidermal growth factor (EGF) on intestinal injury induced by dextran sodium sulfate (DSS) in mice. Twenty-four 6-week-old BALB/c mice were randomly divided into three groups: normal control group and DSS model control group, DSS EGF group. The mice in the normal control group drank tap water, the mice in the DSS model control group drank 5%DSS aqueous solution on the 1st to 7th day of the experiment, and the mice in the; DSS EGF group drank tap water on the 8th to 10th day according to the DSS model control group. At the same time, EGF was injected subcutaneally twice a day for 10 days. The results showed that: 1) compared with the normal control group, the colon length of the DSS model control group was significantly lower than that of the normal control group (P 0.01), and the colon length of the, DSS EGF group was significantly higher than that of the DSS model control group (P 0.01). 2) typical ulcers were found in the colon of the DSS model control group, and the (CDS) score of colon injury degree was significantly higher than that of the normal control group (P 0.01). There was no ulcer in colon tissue of mice in DSS EGF group. Compared with DSS model control group, CDS was significantly decreased (P 0.01). 3) compared with normal control group, the concentration of colon tight junction protein (Occludin) in DSS model control group was significantly lower than that in normal control group (P 0.05). Compared with the DSS model control group, the colon Occludin concentration in the, DSS EGF group was significantly higher than that in the normal control group (P 0.05). Compared with the normal control group, the concentrations of IL-2 (IL-2) and IL-4 (IL-4) in the colon of the DSS model control group were significantly lower than those in the control group (P 0.01), and the concentrations of IL-10 (IL-10) in the control group were significantly lower than those in the normal control group (P 0.05). Compared with the DSS model control group, the concentrations of IL-2 and IL-4 in the colon of, DSS EGF group were significantly increased (P 0.01), and the concentrations of IL-10 were significantly increased (P 0.05). It can be seen that EGF may repair damaged intestinal tissue and maintain the integrity of intestinal mucosal barrier by increasing the expression of Occludin in intestinal tract and regulating the concentration of intestinal cytokines to normal level.
【作者单位】：浙江省农业科学院畜牧兽医研究所;
【基金】：国家现代农业产业技术体系建设专项资金(CARS-36) 国家青年科学基金项目(C170105/31501966) 公益性行业(农业)科研专项(201403047)
【分类号】：S852.3

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