阿塔峡鸡与湖北地方鸡遗传关系的微卫星标记分析

发布时间：2019-07-29 19:12

【摘要】：为分析阿塔峡鸡的遗传结构及其与7个湖北地方鸡的群体遗传关系,利用20个微卫星标记分析了上述8个群体的有效等位基因数(Ne)、期望杂合度(He)、多态信息含量(PIC)及群体间的遗传距离,并进行了聚类分析。结果显示:所检测的20个微卫星位点中LEI0192的等位基因最多(24个),位点MCW0078和ADL210等位基因数最少(10个);检测的8个群体中阿塔峡鸡的期望杂合度和多态信息含量最低(分别为0.6333和0.6178),江汉鸡的最高(分别为0.8475和0.8192);景阳鸡与江汉鸡的遗传距离最远,而阿塔峡鸡与景阳鸡的遗传距离最近;聚类结果显示江汉鸡和双莲鸡聚为一类,郧阳大鸡和洪山鸡聚为一类,然后这4个群体再聚为一大类,随后再与麻城绿壳蛋鸡、荆门黑羽绿壳蛋鸡、阿塔峡鸡依次聚为一类,最后与景阳鸡聚为一类,该结果与这8个群体的外貌特征及品种形成历史基本一致。综上,阿塔峡鸡与景阳鸡的亲缘关系最近,与江汉鸡的亲缘关系最远。
[Abstract]:In order to analyze the genetic structure of Ataxia chicken and its genetic relationship with 7 Hubei local chickens, 20 microsatellite markers were used to analyze the expected heterozygosity (He), polymorphism information content (PIC) and genetic distance between the above 8 populations by using 20 microsatellite markers, and cluster analysis was carried out. The results showed that the most of the 20 microsatellite loci were LEI0192 (24) and the lowest number of MCW0078 and ADL210 loci (10), and the expected heterozygosity and polymorphism information content of Ataxia chicken was the lowest (0.6333 and 0.6178, respectively), and that of Jianghan chicken was the highest (0.8475 and 0.8192, respectively), and the expected heterozygosity and polymorphism information content of Ataxia chicken were the lowest (0.8475 and 0.8192, respectively), and the expected heterozygosity and polymorphism information content of Ataxia chicken were the lowest (0.8475 and 0.8192, respectively). Jingyang chicken and Jianghan chicken have the farthest genetic distance, while Ataxia chicken and Jingyang chicken have the closest genetic distance. The cluster results showed that Jiang Han chicken and Shuanglian chicken were grouped into one group, Yunyang chicken and Hongshan chicken were grouped into one group, and then the four groups were regrouped into a large group, followed by one group with Macheng green shell laying hens, Jingmen black feather green shell laying hens and Ataxia hens, and finally with Jingyang chickens. The results were basically consistent with the appearance characteristics and breed formation history of these eight groups. In summary, the relationship between Ataxia chicken and Jingyang chicken is the closest, and the kinship relationship with Jianghan chicken is the furthest.
【作者单位】：湖北省农业科学院畜牧兽医研究所湖北省农业科技创新中心;动物胚胎工程与分子育种湖北省重点实验室;湖北省京山县畜牧技术推广站;湖北省建始县畜牧技术推广站;
【基金】：动物胚胎工程与分子育种湖北省重点实验室开放课题(2017ZD106) 湖北省农业科技创新中心项目(2016-620-000-001-023) 现代农业产业技术体系建设专项资金(CARS-40-S15)
【分类号】：S831.2

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